Heterocyclic quinones as pharmaceutical agents

ABSTRACT

Pyrrolylquinones and indolylquinones useful for treating diseases such as neurodegenerative disease, viral infections and proliferative disease are described, along with methods of making such compounds and pharmaceutical formulations containing such compounds.

FIELD OF THE INVENTION

This invention relates to synthetic methods for the preparation of pyrrolylquinones and indolylquinones, the compounds so prepared, and uses thereof in the treatment of disease.

BACKGROUND OF THE INVENTION

Demethylasterriquinone is a natural product that was discovered by Merck to have the ability to activate the insulin receptor, and thereby to act orally in glucose lowering in mouse models of diabetes. They have also shown that this compound can activate the TrkA nerve growth factor (neutrophin) receptor. They have further developed a synthetic version identified as “Compound 2” below. See generally Zhang et al., Science 284, 974–977 (1999); Liu et al., J. Med. Chem. 43, 3487–3494 (2000).

U.S. Pat. No. 5,786,488 to Tang et al. proposes synthesis of mono- and bis-indolylquinones using alkali conditions and metal carbonates.

Harris, et al., A One-Pot, Two-Step Synthesis of Tetrahydro Asterriquinone E, Organic Letters 1999, 1(3):431–433 discusses a procedure similar to the '488 patent to produce asterriquinone E.

U.S. Pat. No. 5,780,496 to Tang et al. proposes the utility of indolylquinones as agents for treatment of protein tyrosine kinase cell proliferative disorders.

U.S. Pat. No. 6,011,058 to Zalkow et al. proposes targeting Cdc25 with seco-cholestane derivatives.

Ono et al., Inhibition of HIV-Reverse Transcriptase Activity by Asterriquinone and its Analogues, Biochem. Biophys. Res. Commun. 1991, 174(1):56–62, discusses the ability of asterriquinone and asterriquinone derivatives B1-4, C1-1 and D-1 to inhibit HIV-reverse transcriptase.

U.S. Pat. No. 6,376,529 to Tang et al. proposes the use of monoindolylquinones and bisindolylquinones to treat PTK-related cell proliferative disorders and diabetes mellitus.

SUMMARY OF THE INVENTION

A first aspect of the present invention is an acid-catalyzed method of producing a compound of Formula I:

wherein:

R₁ and R₃ are each independently hydrogen, OH, SH, halo, amino, alkoxy, aminoalkyl, alkyl, aryl, acyloxy, or (acyloxy)alkyl

R₂ is hydrogen, aryl, heteroaryl, indolyl, alkyl, alkoxy, phenoxy, anilino, amino, halo, acyloxy, or (acyloxy)alkyl;

or R₁ and R₂ together form an aromatic ring (e.g., R1 and R2 together form —CH═CH—CH—, —CH═CH—CH═CH—, or —CH═CH—CH═CH—CH═CH—);

R₅ is hydrogen, C₁–C₇ alkyl, C₂–C₇ alkenyl, C₂–C₇ alkynyl, arylalkyl, or aryl;

R₄ is hydrogen, branched or unbranched saturated C₁–C_(n) alkyl, branched or unbranched unsaturated C₁–C_(n) alkyl, alkylcarboxy, C₂–C_(m) alkenyl, alkynyl, alkenylcarboxy, aryl, alkylaryl hydroxy, hydroxyalkyl, C₁–C_(n) alkoxy, nitro, halo, trihalomethyl, amido, carboxamido, carboxy, sulfonyl, sulfonamido, amino, mercapto, or 2-methylbut-2-en-4-yl, wherein n is an integer from 2–12, preferably 2–7, and m is an integer from 3–12, preferably 3–7; and

R₆ and R₇ are each independently hydrogen, cycloalkyl, alkyl, alkoxy, halo, aryl, heteroaryl, phenoxy, anilino, amino, or form part of an aromatic ring ((e.g., R6 and R7 together form —CH═CH—CH—, —CH═CH—CH═CH—, or —CH═CH—CH═CH—CH═CH—) wherein said aromatic ring may be unsubstituted or substituted 1, 2 or 3 times with cycloalkyl, alkyl, alkoxy, halo, aryl, heteroaryl, phenoxy, anilino, amino;

which method comprises:

reacting a substituted or unsubstituted 2,5-dichloro-1,4-benzoquinone compound of the formula:

wherein R₁, R₂ and R₃ are as defined above;

with at least one pyrrole of the formula:

wherein R₄–R₇ are as defined above,

in a polar organic solvent and in the presence of an acid to produce a first intermediate; and then

reacting the first intermediate with an oxidization agent to produce said compound of formula I. In some embodiments of the foregoing, n is 2–7 and m is 3–7. In some embodiments, the method further comprises: reacting said compound of formula I with an alkali metal hydroxide to produce a compound of the formula:

wherein R₂ and R₄–R₇ are as defined above. The organic solvent may be, for example, an aprotic solvent selected from the group consisting of tetrahydrofuran (THF), acetonitrile, and mixtures thereof. In some embodiments, the acid may be HCl, H₂SO₄, AcOH (acetic acid), or mixtures thereof. In some embodiments the oxidization agent is dichlorodicyanobenzoquinone, Ag₂CO₃, or mixtures thereof. The reaction may be conducted at any suitable temperature, such as from about −10° C. to about 100° C.

A second aspect of the present invention is an acid-catalyzed method of producing a compound of formula V:

wherein:

R₁ and R₃ are each independently OH, SH, halo, amino, alkoxy, aminoalkyl, hydrogen, alkyl, aryl, acyloxy, or (acyloxy)alkyl;

R₂ is hydrogen, aryl, heteroaryl, indolyl, alkyl, alkoxy, phenoxy, anilino, amino, halo, acyloxy, or (acyloxy)alkyl; or

R₁ and R₂ can constitute part of an aromatic ring (e.g., R1 and R2 together form —CH═CH—CH—, —CH═CH—CH═CH—, or —CH═CH—CH═CH—CH═CH—);

R₅ is hydrogen, C₁–C₇ alkyl, C₂–C₇ alkenyl, C₂–C₇ alkynyl, arylalkyl, or aryl; and

R₄ and R₆–R₉ are each independently hydrogen, branched or unbranched C₁–C_(n) alkyl, alkylcarboxy, C₂–C_(m) alkenyl, alkynyl, alkenylcarboxy, aryl, alkylaryl, hydroxy, hydroxyalkyl, C₁–C_(n) alkoxy, nitro, halo, trihalomethyl, amido, carboxamido, carboxy, sulfonyl, sulfonamido, amino, mercapto, or 2-methylbut-2-en-4-yl, wherein n is an integer from 2–12, and m is an integer from 3–12;

which method comprises:

reacting a substituted or unsubstituted 2,5-dichloro-1,4-benzoquinone compound of the formula:

wherein R₁, R₂ and R₃ are as defined above;

with at least one indole of the formula:

wherein R₄–R₉ are as defined above;

in a polar organic solvent and in the presence of an acid to produce a first intermediate; and then

reacting the first intermediate with an oxidization agent to produce said compound of formula V. In some embodiments, n is 2–7 and m is 3–7. The method may further comprise reacting said compound of formula I with an alkali metal hydroxide to produce a compound of the formula:

wherein R₂ and R₄–R₉ are as defined above. In some embodiments, the organic solvent is an aprotic solvent selected from the group consisting of tetrahydrofuran (THF), acetonitrile, and mixtures thereof. The acid may, for example, be HCl, H₂SO₄, AcOH, or mixtures thereof. The oxidization agent may be dichlorodicyanobenzoquinone, Ag₂CO₃ or combinations thereof. The reaction may in some embodiments be conducted at a temperature from about −10° C. to about 100° C.

A further aspect of the present invention is a compound of the formula I:

wherein:

R₁ and R₃ are each independently hydrogen, OH, SH, halo, amino, alkoxy, aminoalkyl, alkyl, aryl, acyloxy, or (acyloxy)alkyl;

R₂ is hydrogen, aryl, heteroaryl, indolyl, alkyl, alkoxy, phenoxy, anilino, amino, halo, acyloxy, or (acyloxy)alkyl;

or R₁ and R₂ together form an aromatic ring (e.g., R1 and R2 together form —CH═CH—CH—, —CH═CH—CH═CH—, or —CH═CH—CH═CH—CH═CH—);

R₅ is hydrogen, C₁–C₇ alkyl, C₂–C₇ alkenyl, C₂–C₇ alkynyl, arylalkyl, or aryl;

R₄ is hydrogen, branched or unbranched saturated C₁–C_(n) alkyl, branched or unbranched unsaturated C₁–C_(n) alkyl, alkylcarboxy, C₂–C_(m) alkenyl, alkynyl, alkenylcarboxy, aryl, alkylaryl hydroxy, hydroxyalkyl, C₁–C_(n) alkoxy, nitro, halo, trihalomethyl, amido, carboxamido, carboxy, sulfonyl, sulfonamido, amino, mercapto, or 2-methylbut-2-en-4-yl, wherein n is an integer from 2–7 or 12, and m is an integer from 3–7 or 12; and

R₆ and R₇ are each independently hydrogen, cycloalkyl, alkyl, alkoxy, halo, aryl, heteroaryl, phenoxy, anilino, amino, or form part of an aromatic ring (e.g., R6 and R7 together form —CH═CH—CH—, —CH═CH—CH═CH—, or —CH═CH—CH═CH—CH═CH—) wherein said aromatic ring is unsubstituted or substituted 1, 2 or 3 times with cycloalkyl, alkyl, alkoxy, halo, aryl, heteroaryl, phenoxy, anilino, or amino.

A further aspect of the present invention is a compound of the formula V:

wherein:

R₁ and R₃ are each independently OH, SH, halo, amino, alkoxy, aminoalkyl, hydrogen, alkyl, aryl, acyloxy, or (acyloxy)alkyl;

R₂ is hydrogen, aryl, heteroaryl, indolyl, alkyl, alkoxy, phenoxy, anilino, amino, halo, acyloxy, or (acyloxy)alkyl;

R₁ and R₂ can constitute part of an aromatic ring (e.g., R1 and R2 together form —CH═CH—CH—, —CH═CH—CH═CH—, or —CH═CH—CH═CH—CH═CH—).

R₅ is hydrogen, C₁–C₇ alkyl, C₂–C₇ alkenyl, C₂–C₇ alkynyl, arylalkyl, or aryl; and

R₄ and R₆–R₉ are each independently hydrogen, branched or unbranched C₁–C_(n) alkyl, alkylcarboxy, C₂–C_(m) alkenyl, alkynyl, alkenylcarboxy, aryl, alkylaryl, hydroxy, hydroxyalkyl, C₁–C_(n) alkoxy, nitro, halo, trihalomethyl, amido, carboxamido, carboxy, sulfonyl, sulfonamido, amino, mercapto, or 2-methylbut-2-en-4-yl, wherein n is an integer from 2 to 7 or 12, and m is an integer from 3 to 7 or 12.

A further aspect of the present invention is a method of treating a proliferative disease in a subject in need thereof, comprising administering to said subject, in an amount effective to treat said proliferative disease, a compound of formula I or V as given above. Examples of such said proliferative diseases include but are not limited to ovarian cancer, breast cancer, colon cancer, gastric carcinomas, non-small cell lung cancer, and non-Hodgkin's lymphoma.

A still further aspect of the present invention is a method for treating a viral infection in a subject so afflicted, said method comprising administering to the subject a compound of the formula I or V as given above in an amount effective to treat the viral disease. Examples of infections which may be treated include Poxviridae, Filoviridae, Herpesviridae, Hepadnaviridae, and Retroviridae infections.

A still further aspect of the present invention is a method for treating a neurodegenerative disease in a subject so afflicted, said method comprising administering to the subject a compound of the formula I or V as given above in an amount effective to treat said neurodegenerative disease. Examples of neurodegenerative diseases which may be so treated include but are not limited to Alzheimer's disease, Parkinson's disease, multiple sclerosis, and amyotropic lateral sclerosis.

A still further aspect of the present invention is the use of a compound of Formula I or Formula V (an active agent) as described above for the preparation of a medicament for the treatment of a disorder as described above.

The present invention is explained in greater detail in the specification set forth below.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

The term “treat” as used herein refers to any type of treatment that imparts a benefit to a patient afflicted with a disease, including improvement in the condition of the patient (e.g., in one or more symptoms), delay in the progression of the disease, etc.

The term “aryl” refers to an aromatic group or substituent whose molecules have the ring structure characteristic of benzene, naphthalene, phenanthrene, anthracene, etc. (i.e., either the 6-carbon ring of benzene or the condensed 6-carbon rings of the other aromatic derivatives). For example, an aryl group may be phenyl (C₆H₅), naphthyl (C₁₀H₇), etc. Aryl groups may be unsubstituted or substituted one, two, three or more times with additional substituents as described below (e.g., alkyl, halo, alkoxy, etc.).

“Heteroaryl” means an unsubstituted or substituted 5- or 6-membered monocyclic hetereoaromatic ring or a 9- or 10-membered bicyclic hetereoaromatic ring containing 1, 2, 3 or 4 hetereoatoms which are independently N, S or O. Examples of hetereoaryl rings are furan, thiophene, pyrrole, pyridine, benzimidazole, indole, imidazole, isoquinoline, quinzoline and the like, the corresponding functional groups of which are al examples of heteroaryl groups that may be used in the present invention. Substituents on heteroaryl groups include those other as defined herein are included in the definition of heteroaryl.

“Alkyl” as used herein refers to a paraffinic hydrocarbon group which may be derived from an alkane by dropping one hydrogen from the formula. Examples are methyl, ethyl, propyl, isopropyl, butyl, tert-butyl, etc. Unless otherwise specified herein, “alkyl” as used herein is preferably a loweralkyl, e.g., C1–C4 loweralkyl. “Alkyl” groups as used herein may be saturated or unsaturated, although the unsaturated alkyls may be identified as “alkenyl” or “alkynyl” groups as described below.

“Cycloalkyl” as used herein means an unsubstituted or substituted 3- to 7-membered carbacyclic ring, which may be unsubstituted or substituted (e.g., from 3 to 7 times) with substituents as described herein.

“Alkenyl” as used herein refers to an alkyl group as defined above modified to contain at least one double bond (e.g., at least one degree of unsaturation). Unless otherwise specified herein, “alkenyl” as used herein is preferably a loweralkenyl, e.g., C1–C4 loweralkenyl.

“Alkynyl” as used herein refers to an alkyl group as defined above modified to contain at least one triple bond. Unless otherwise specified herein, “alkynyl” as used herein is preferably a loweralkynyl, e.g., C1–C4 loweralkynyl.

“Alkoxy” as used herein means a substituent of the formula —OR, where R is alkyl as defined above.

“Halo” as used herein refers to one of the electronegative elements of group VIIA of the periodic table (e.g., fluoro, chloro bromo, iodo).

“Amino” refers to the —NH₂ group.

“Aminoalkyl” refers to a group of the formula —NRR′, where at least one, or both, of R and R′ are alkyl as defined above. One of R and R′ may optionally be H.

“Acyl” refers to an organic acid group in which the OH of the carboxyl group is removed so that a bond may be formed. Thus an acyl group may be generally represented by the formula RCO—, where R is a substituent as described herein (e.g., alkyl, aryl, arylalkyl), O is double bonded to the carbon, and the bond drawn in the structure is to the carbon shown in the structure). Examples include, but are not limited to acetyl, benzoyl, etc.

“Acyloxy” as used herein refers to a group of the formula —OR′, where R′ is acyl as defined above.

“(Acyloxy)alkyl” as used herein refers to a group of the formula —R—OR′, where R′ is acyl as defined above and R is alkyl as defined above.

“Arylalkyl” as used herein refers to a group of the formula —RR″, where R″ is aryl as defined above and R is alkyl as defined above. An example is benzyl.

The term “pharmaceutically acceptable” as used herein means that the compound or composition is suitable for administration to a subject to achieve the treatments described herein, without unduly deleterious side effects in light of the severity of the disease and necessity of the treatment.

The present invention is primarily concerned with the treatment of human subjects, but the invention may also be carried out on animal subjects, particularly mammalian subjects such as mice, rats, dogs, cats, livestock and horses for veterinary purposes, and for drug screening and drug development purposes.

1. Active Compounds

The methods of the present invention include the administration of compounds of Formula I, while pharmaceutical compositions of the present invention comprise compounds of Formula I as described above.

Compounds illustrative of the compounds of Formula (I) above include:

2-tert-Butyl-3,6-dihydroxy-5-(5-phenethyl-4-phenyl-1H-pyrrol-3-yl)-[1,4]benzoquinone;

Acetic acid 4-acetoxymethoxy-2-(5-benzyloxymethyl-4-methyl-1H-pyrrol-3-yl)-3,6-dioxo-5-phenyl-cyclohexa-1,4-dienyloxymethyl ester; and

Acetic acid 4-acetoxy-2-(2-cyclopropyl-4,5,6,7-tetrahydro-1H-indol-3-yl)-5-methyl-3,6-dioxo-cyclohexa-1,4-dienyl ester.

The methods of the present invention include the administration of compounds of Formula V, while pharmaceutical compositions of the present invention comprise compounds of Formula V as given above. Compounds illustrative of the compounds of Formula (V) above include: 2,5-Dichloro-3-(1H-indol-3-yl)-[1,4]benzoquinone; 2,5-Dichloro-3-(2-metthyl-1H-indol-3-yl)-[1,4]benzoquinone; 2,5-Dichloro-3-(2,5dimethyl-1H-indol-3-yl)-[1,4]benzoquinone; 2,5-Dichloro-3-(5-methoxy-2-methyl-1H-indol-3-yl)-[1,4]benzoquinone; 2,5-Dichloro-3-(5-chloro-2-methyl-1H-indol-3-yl)-[1,4]benzoquinone; 2,5-Dichloro-3-(2-cyclopropyl-1H-indol-3-yl)-[1,4]benzoquinone; 2,5-Dichloro-3-(2-phenyl-1H-indol-3-yl)-[1,4]benzoquinone; 2,5-Dichloro-3-(1-methyl-1H-indol-3-yl)-[1,4]benzoquinone; 2,5-Dichloro-3-(2-ethyl-1H-indol-3-yl)-[1,4]benzoquinone; 2,5-Dichloro-3-(2-isopropyl-1H-indol-3-yl)-[1,4]benzoquinone; 2,5-Dichloro-3-(2-tert-butyl-1H-indol-3-yl)-[1,4]benzoquinone; 2,5-Dichloro-3-[2-(1-methyl-cyclopropyl)-1H-indol-3-yl]-[1,4]benzoquinone; 2,5-Dichloro-3-[2-(1-methyl-cyclohexyl)-1H-indol-3-yl]-[1,4]benzoquinone; 3-(4-Fluoro-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone; 3-(4-Chloro-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone; 3-(4-Bromo-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone; 2,5-Dichloro-3-(4-methoxy-1H-indol-3-yl)-[1,4]benzoquinone; 3-(4-Benzyloxy-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone; 2,5-dichloro-3-(4-methyl-1H-indol-3-yl)-[1,4]benzoquinone; 3-(5-Fluoro-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone; 3-(5-Chloro-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone; 3-(5-Bromo-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone; 2,5-Dichloro-3-(5-hydroxy-1H-indol-3-yl)-[1,4]benzoquinone; 2,5-Dichloro-3-(5-methoxy-1H-indol-3-yl)-[1,4]benzoquinone; 3-(5-Benzyloxy-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone; 2,5-Dichloro-3-(5-methyl-1H-indol-3-yl)-[1,4]benzoquinone; 3-(6-Fluoro-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone; 3-(6-Chloro-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone; 3-(6-Bromo-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone; 2,5-Dichloro-3-(6-methyl-1H-indol-3-yl)-[1,4]benzoquinone; 2,5-Dichloro-3-(7-propyl-1H-indol-3-yl)-[1,4]benzoquinone; 2,5-Dichloro-3-(7-methyl-1H-indol-3-yl)-[1,4]benzoquinone; 3-(7-tert-butyl-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone; 2,5-Dichloro-3-(7-phenyl-1H-indol-3-yl)-[1,4]benzoquinone; 2,5-Dichloro-3-(7-methoxy-1H-indol-3-yl)-[1,4]benzoquinone; 3-(7-Benzyloxy-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone; 3-(7-Fluoro-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone; 3-(7-Chloro-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone; 3-(7-Bromo-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone; 3-(7-Benzyl-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone; 2,5-Dichloro-3-[7-(2-methyl-benzyl)-1H-indol-3-yl]-[1,4]benzoquinone; 3-(1H-Benzo[g]indol-3-yl)-2,5-dichloro-[1,4]benzoquinone; 3-(2,6-Dimethyl-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone; 3-(2,7-Dimethyl-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone; 3-(6,7-Dimethyl-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone; 3-(5,6-Methylenedioxy-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone; 3-(5,6-Dimethoxy-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone; 2,5-Dihydroxy-3-(1H-indol-3-yl)-[1,4]benzoquinone; 2,5-Dihydroxy-3-(2-metthyl-1H-indol-3-yl)-[1,4]benzoquinone; 2,5-Dihydroxy-3-(2,5dimethyl-1H-indol-3-yl)-[1,4]benzoquinone; 2,5-Dihydroxy-3-(5-methoxy-2-methyl-1H-indol-3-yl)-[1,4]benzoquinone; 2,5-Dihydroxy-3-(5-chloro-2-methyl-1H-indol-3-yl)-[1,4]benzoquinone; 2,5-Dihydroxy-3-(2-cyclopropyl-1H-indol-3-yl)-[1,4]benzoquinone; 2,5-Dihydroxy-3-(2-phenyl-1H-indol-3-yl)-[1,4]benzoquinone; 2,5-Dihydroxy-3-(1-methyl-1H-indol-3-yl)-[1,4]benzoquinone; 2,5-Dihydroxy-3-(2-ethyl-1H-indol-3-yl)-[1,4]benzoquinone; 2,5-Dihydroxy-3-(2-isopropyl-1H-indol-3-yl)-[1,4]benzoquinone; 2,5-Dihydroxy-3-(2-tert-butyl-1H-indol-3-yl)-[1,4]benzoquinone; 2,5-Dihydroxy-3-[2-(1-methyl-cyclopropyl)-1H-indol-3-yl]-[1,4]benzoquinone; 2,5-Dihydroxy-3-[2-(1-methyl-cyclohexyl)-1H-indol-3-yl]-[1,4]benzoquinone; 3-(4-Fluoro-1H-indol-3-yl)-2,5-dihydroxy-[1,4]benzoquinone; 3-(4-Chloro-1H-indol-3-yl)-2,5-dihydroxy-[1,4]benzoquinone; 3-(4-Bromo-1H-indol-3-yl)-2,5-dihydroxy-[1,4]benzoquinone; 2,5-Dihydroxy-3-(4-methoxy-1H-indol-3-yl)-[1,4]benzoquinone; 3-(4-Benzyloxy-1H-indol-3-yl)-2,5-dihydroxy-[1,4]benzoquinone; 2,5-dihydroxy-3-(4-methyl-1H-indol-3-yl)-[1,4]benzoquinone; 3-(5-Fluoro-1H-indol-3-yl)-2,5-dihydroxy-[1,4]benzoquinone; 3-(5-Chloro-1H-indol-3-yl)-2,5-dihydroxy-[1,4]benzoquinone; 3-(5-Bromo-1H-indol-3-yl)-2,5-dihydroxy-[1,4]benzoquinone; 2,5-Dihydroxy-3-(5-hydroxy-1H-indol-3-yl)-[1,4]benzoquinone; 2,5-Dihydroxy-3-(5-methoxy-1H-indol-3-yl)-[1,4]benzoquinone; 3-(5-Benzyloxy-1H-indol-3-yl)-2,5-dihydroxy-[1,4]benzoquinone; 2,5-Dihydroxy-3-(5-methyl-1H-indol-3-yl)-[1,4]benzoquinone; 3-(6-Fluoro-1H-indol-3-yl)-2,5-dihydroxy-[1,4]benzoquinone; 3-(6-Chloro-1H-indol-3-yl)-2,5-dihydroxy-[1,4]benzoquinone; 3-(6-Bromo-1H-indol-3-yl)-2,5-dihydroxy-[1,4]benzoquinone; 2,5-Dihydroxy-3-(6-methyl-1H-indol-3-yl)-[1,4]benzoquinone; 2,5-Dihydroxy-3-(7-propyl-1H-indol-3-yl)-[1,4]benzoquinone; 2,5-Dihydroxy-3-(7-methyl-1H-indol-3-yl)-[1,4]benzoquinone; 3-(7-tert-butyl-1H-indol-3-yl)-2,5-dihydroxy-[1,4]benzoquinone 2,5-Dihydroxy-3-(7-phenyl-1H-indol-3-yl)-[1,4]benzoquinone; 2,5-Dihydroxy-3-(7-methoxy-1H-indol-3-yl)-[1,4]benzoquinone; 3-(7-Benzyloxy-1H-indol-3-yl)-2,5-dihydroxy-[1,4]benzoquinone; 3-(7-Fluoro-1H-indol-3-yl)-2,5-dihydroxy-[1,4]benzoquinone; 3-(7-Chloro-1H-indol-3-yl)-2,5-dihydroxy-[1,4]benzoquinone; 3-(7-Bromo-1H-indol-3-yl)-2,5-dihydroxy-[1,4]benzoquinone; 3-(7-Benzyl-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone; 2,5-Dihydroxy-3-[7-(2-methyl-benzyl)-1H-indol-3-yl]-[1,4]benzoquinone; 3-(1H-Benzo[g]indol-3-yl)-2,5-dihydroxy-[1,4]benzoquinone; 3-(2,6-Dimethyl-1H-indol-3-yl)-2,5-dihydroxy-[1,4]benzoquinone; 3-(2,7-Dimethyl-1H-indol-3-yl)-2,5-dihydroxy-[1,4]benzoquinone; 3-(6,7-Dimethyl-1H-indol-3-yl)-2,5-dihydroxy-[1,4]benzoquinone; 3-(5,6-Methylenedioxy-1H-indol-3-yl)-2,5-dihydroxy-[1,4]benzoquinone; and 3-(5,6-Dimethoxy-1H-indol-3-yl)-2,5-dihydroxy-[1,4]benzoquinone.

The active compounds disclosed herein can, as noted above, be prepared in the form of their pharmaceutically acceptable salts. Pharmaceutically acceptable salts are salts that retain the desired biological activity of the parent compound and do not impart undesired toxicological effects. Examples of such salts are (a) acid addition salts formed with inorganic acids, for example hydrochloric acid, hydrobromic acid, sulfuric acid, phosphoric acid, nitric acid and the like; and salts formed with organic acids such as, for example, acetic acid, oxalic acid, tartaric acid, succinic acid, maleic acid, fumaric acid, gluconic acid, citric acid, malic acid, ascorbic acid, benzoic acid, tannic acid, palmitic acid, alginic acid, polyglutamic acid, naphthalenesulfonic acid, methanesulfonic acid, p-toluenesulfonic acid, naphthalenedisulfonic acid, polygalacturonic acid, and the like; (b) salts formed from elemental anions such as chlorine, bromine, and iodine, and (c) salts derived from bases, such as ammonium salts, alkali metal salts such as those of sodium and potassium, alkaline earth metal salts such as those of calcium and magnesium, and salts with organic bases such as dicyclohexylamine and N-methyl-D-glucamine.

2. Pharmaceutical Formulations

The active compounds described above may be formulated for administration in a pharmaceutical carrier in accordance with known techniques. See, e.g., Remington, The Science And Practice of Pharmacy (9^(th) Ed. 1995). In the manufacture of a pharmaceutical formulation according to the invention, the active compound (including the physiologically acceptable salts thereof) is typically admixed with, inter alia, an acceptable carrier. The carrier must, of course, be acceptable in the sense of being compatible with any other ingredients in the formulation and must not be deleterious to the patient. The carrier may be a solid or a liquid, or both, and is preferably formulated with the compound as a unit-dose formulation, for example, a tablet, which may contain from 0.01 or 0.5% to 95% or 99% by weight of the active compound. One or more active compounds may be incorporated in the formulations of the invention, which may be prepared by any of the well known techniques of pharmacy consisting essentially of admixing the components, optionally including one or more accessory ingredients.

The formulations of the invention include those suitable for oral, rectal, topical, buccal (e.g., sub-lingual), vaginal, parenteral (e.g., subcutaneous, intramuscular, intradermal, or intravenous), topical (i.e., both skin and mucosal surfaces, including airway surfaces) and transdermal administration, although the most suitable route in any given case will depend on the nature and severity of the condition being treated and on the nature of the particular active compound which is being used.

Preferred routes of parenteral administration include intrathecal injection, including directly into the tumor, and intraventricular injection into a ventricle of the brain.

Formulations suitable for oral administration may be presented in discrete units, such as capsules, cachets, lozenges, or tablets, each containing a predetermined amount of the active compound; as a powder or granules; as a solution or a suspension in an aqueous or non-aqueous liquid; or as an oil-in-water or water-in-oil emulsion. Such formulations may be prepared by any suitable method of pharmacy which includes the step of bringing into association the active compound and a suitable carrier (which may contain one or more accessory ingredients as noted above). In general, the formulations of the invention are prepared by uniformly and intimately admixing the active compound with a liquid or finely divided solid carrier, or both, and then, if necessary, shaping the resulting mixture. For example, a tablet may be prepared by compressing or molding a powder or granules containing the active compound, optionally with one or more accessory ingredients. Compressed tablets may be prepared by compressing, in a suitable machine, the compound in a free-flowing form, such as a powder or granules optionally mixed with a binder, lubricant, inert diluent, and/or surface active/dispersing agent(s). Molded tablets may be made by molding, in a suitable machine, the powdered compound moistened with an inert liquid binder.

Formulations suitable for buccal (sub-lingual) administration include lozenges comprising the active compound in a flavoured base, usually sucrose and acacia or tragacanth; and pastilles comprising the compound in an inert base such as gelatin and glycerin or sucrose and acacia.

Formulations of the present invention suitable for parenteral administration comprise sterile aqueous and non-aqueous injection solutions of the active compound, which preparations are preferably isotonic with the blood of the intended recipient. These preparations may contain anti-oxidants, buffers, bacteriostats and solutes which render the formulation isotonic with the blood of the intended recipient. Aqueous and non-aqueous sterile suspensions may include suspending agents and thickening agents. The formulations may be presented in unit\dose or multi-dose containers, for example sealed ampoules and vials, and may be stored in a freeze-dried (lyophilized) condition requiring only the addition of the sterile liquid carrier, for example, saline or water-for-injection immediately prior to use. Extemporaneous injection solutions and suspensions may be prepared from sterile powders, granules and tablets of the kind previously described. For example, in one aspect of the present invention, there is provided an injectable, stable, sterile composition comprising a compound of Formula (I), or a salt thereof, in a unit dosage form in a sealed container. The compound or salt is provided in the form of a lyophilizate which is capable of being reconstituted with a suitable pharmaceutically acceptable carrier to form a liquid composition suitable for injection thereof into a subject. The unit dosage form typically comprises from about 10 mg to about 10 grams of the compound or salt. When the compound or salt is substantially water-insoluble, a sufficient amount of emulsifying agent which is physiologically acceptable may be employed in sufficient quantity to emulsify the compound or salt in an aqueous carrier. One such useful emulsifying agent is phosphatidyl choline.

Formulations suitable for rectal administration are preferably presented as unit dose suppositories. These may be prepared by admixing the active compound with one or more conventional solid carriers, for example, cocoa butter, and then shaping the resulting mixture.

Formulations suitable for topical application to the skin preferably take the form of an ointment, cream, lotion, paste, gel, spray, aerosol, or oil. Carriers which may be used include petroleum jelly, lanoline, polyethylene glycols, alcohols, transdermal enhancers, and combinations of two or more thereof.

Formulations suitable for transdermal administration may be presented as discrete patches adapted to remain in intimate contact with the epidermis of the recipient for a prolonged period of time. Formulations suitable for transdermal administration may also be delivered by iontophoresis (see, for example, Pharmaceutical Research 3 (6):318 (1986)) and typically take the form of an optionally buffered aqueous solution of the active compound. Suitable formulations comprise citrate or bis\tris buffer (pH 6) or ethanol/water and contain from 0.1 to 0.2M active ingredient.

Further, the present invention provides liposomal formulations of the compounds disclosed herein and salts thereof. The technology for forming liposomal suspensions is well known in the art. When the compound or salt thereof is an aqueous-soluble salt, using conventional liposome technology, the same may be incorporated into lipid vesicles. In such an instance, due to the water solubility of the compound or salt, the compound or salt will be substantially entrained within the hydrophilic center or core of the liposomes. The lipid layer employed may be of any conventional composition and may either contain cholesterol or may be cholesterol-free. When the compound or salt of interest is water-insoluble, again employing conventional liposome formation technology, the salt may be substantially entrained within the hydrophobic lipid bilayer which forms the structure of the liposome. In either instance, the liposomes which are produced may be reduced in size, as through the use of standard sonication and homogenization techniques.

Of course, the liposomal formulations containing the compounds disclosed herein or salts thereof, may be lyophilized to produce a lyophilizate which may be reconstituted with a pharmaceutically acceptable carrier, such as water, to regenerate a liposomal suspension.

Other pharmaceutical compositions may be prepared from the water-insoluble compounds disclosed herein, or salts thereof, such as aqueous base emulsions. In such an instance, the composition will contain a sufficient amount of pharmaceutically acceptable emulsifying agent to emulsify the desired amount of the compound or salt thereof. Particularly useful emulsifying agents include phosphatidyl cholines, and lecithin.

In addition to compounds of formula (I) or (V) or their salts, the pharmaceutical compositions may contain other additives, such as pH-adjusting additives. In particular, useful pH-adjusting agents include acids, such as hydrochloric acid, bases or buffers, such as sodium lactate, sodium acetate, sodium phosphate, sodium citrate, sodium borate, or sodium gluconate. Further, the compositions may contain microbial preservatives. Useful microbial preservatives include methylparaben, propylparaben, and benzyl alcohol. The microbial preservative is typically employed when the formulation is placed in a vial designed for multidose use. Of course, as indicated, the pharmaceutical compositions of the present invention may be lyophilized using techniques well known in the art.

3. Dosage and Routes of Administration

As noted above, the present invention provides pharmaceutical formulations comprising the active compounds (including the pharmaceutically acceptable salts thereof), in pharmaceutically acceptable carriers for oral, rectal, topical, buccal, parenteral, intramuscular, intradermal, or intravenous, and transdermal administration.

Preferred routes of parenteral administration include intrathecal injection, including directly into the tumor, and intraventricular injection into a ventricle of the brain.

The therapeutically effective dosage of any one active agent, the use of which is in the scope of present invention, will vary somewhat from compound to compound, and patient to patient, and will depend upon factors such as the age and condition of the patient and the route of delivery. Such dosages can be determined in accordance with routine pharmacological procedures known to those skilled in the art.

The therapeutically effective dosage of any specific compound, the use of which is in the scope of present invention, will vary somewhat from compound to compound, and patient to patient, and will depend upon the condition of the patient and the route of delivery. As a general proposition, a dosage from about 0.1 to about 50 mg/kg will have therapeutic efficacy, with all weights being calculated based upon the weight of the active compound, including the cases where a salt is employed. Toxicity concerns at the higher level may restrict intravenous dosages to a lower level such as up to about 10 mg/kg, with all weights being calculated based upon the weight of the active base, including the cases where a salt is employed. A dosage from about 10 mg/kg to about 50 mg/kg may be employed for oral administration. Typically, a dosage from about 0.5 mg/kg to 5 mg/kg may be employed for intramuscular injection. Preferred dosages are 1 μmol/kg to 50 μmol/kg, and more preferably 22 μmol/kg and 33 μmol/kg of the compound for intravenous or oral administration. The duration of the treatment is usually once per day for a period of two to three weeks or until the condition is essentially controlled. Lower doses given less frequently can be used prophylactically to prevent or reduce the incidence of recurrence of the infection.

4. Compounds as Antiviral Agents

There are comparatively fewer antivirals than there are antibiotics. Since viruses engage in much of their infective activity by hijacking a cell's machinery and essentially directing the cell to manufacture virus particles, agents with antiviral effect may additionally inhibit cellular functions in non-infected cells. For example, iododeoxyuridine, one of the first antiviral agents, has significant systemic toxicity.

While advances have lead to more advanced compounds, such as acyclovir and AZT, the number of viruses that are currently targeted by antiviral medicine lags far behind that of antibiotics.

The present invention shows use in several families of viruses, both in traditional antiviral targets and in families that are currently unmet with antiviral medication.

In one embodiment of the invention, compounds of the invention are effective in treating infection by viruses of the family Poxviridae, such as variola (smallpox), vaccinia, goatpox, swinepox. Examples of such compounds include but are not limited to compounds 6C, 6F, 11C, and 8H from Table 3 below.

It is known in the art that asterriquinone may inhibit HIV replication by interacting with viral Reverse Transcriptase. In another embodiment of the invention, compounds of the invention are effective in treating infection by viruses which utilize a Reverse Transcriptase mechanism, namely viruses of the family Retroviridae. Examples of such compounds include but are not limited to the compounds listed in Table 3 below.

Filoviridae infections are characterized by a rapidly acute and often fatal infection in a large percentage of patients. Additionally, Filoviridae, specifically Ebola, are feared biological weapons agents. Compounds embodying this invention may have utility against Filoviridae, such as Ebola and Marburg.

Hepadnaviridae exhibit control over the RAS pathway by utilization of viral protein X. Compounds embodying this invention may have utility against Hepadnaviridae, such as Hepatitis B.

Herpesviridae depend on a viral thymadine kinase pathway, the target of antivirals such as acyclovir. Compounds embodying this invention may have utility against Herpesviridae, such as HSV-1.

The present invention is explained in greater detail in the following non-limiting Examples.

EXAMPLE 1 HCl Preperation of 2,5-dichloro-3-(2-methyl-1H-indol-3-yl)-[1,4]benzoquinone (Procedure A)

To a solution of 2-methylindole (0.500 g, 3.81 mmol) in THF (30 mL) was added 2,5-dichloro-1,4-benzoquinone (1.35 g, 7.62 mmol) at room temperature. To this mixture was added concentrated HCl (12 N, 0.38 mL, 4.57 mmol) dropwise. After the reaction mixture was stirred for 5 hours (5 h–12 h) at room temperature, DDQ (1.73 g, 7.62 mmol) was added. After stirring for 3 hours (3 h–12 h) at room temperature, the mixture was diluted with EtOAc (100 mL). The organic solution was washed with saturated NaHCO₃ (3×100 mL) and brine (100 mL), and dried over Na₂SO₄. The residue was concentrated and purified by flash column chromatography using 15% EtOAc in hexane as eluent to afford pure 2,5-dichloro-3-(2-methyl-1H-indol-3-yl)-[1,4]benzoquinone (1.13 g, 97%) as a blue solid. Melting point 158–159° C. R_(f)=0.35 (3:7, EtOAc/hexane). ¹H NMR (CDCl₃) δ 8.40 (bs, NH), 7.24–7.11 (m, 5H), 2.26 (s, 3H). ¹H NMR (d₆-acetone): δ 10.60 (1H, br s), 7.39 (1H, s), 7.36 (1H, d, J=7.8 Hz), 7.24 (1H, d, J=7.8 Hz), 7.10 (1H, t, J=7.8 Hz), 7.01 (1H, t, J=7.8 Hz). ¹³C NMR (CDCl₃) δ 177.7, 176.6, 144.3, 139.8, 139.3, 136.9, 135.1, 133.1, 126.6, 122.0, 120.5, 119.6, 110.8, 104.8, 13.9. IR (KBr): 3388, 3061, 1676, 1566, 1459, 1250, 1032, 749 cm⁻¹. Anal. Calcd. for C₁₅H₉Cl₂NO₂: C, 58.85; H, 2.96; Cl, 23.16; N, 4.58. Found: C, 58.85; H, 2.96; Cl, 23.16; N, 4.58. HRMS (EI) Calcd. For C₁₅H₉Cl₂NO₂ [M⁺]: 305.0010. Found: 305.0002.

EXAMPLE 2 H₂SO₄ Preperation of 2,5-dichloro-3-(5-methoxy-1H-indol-3-yl)-[1,4]benzoquinone (Procedure B)

To a solution of 2,5-dichloro-1,4-benzoquinone (0.242 g, 1.37 mmol) in THF (3 mL) was added H₂SO₄ (35 μL, 0.68 mmol) at room temperature. To this mixture was added 5-methoxyindole (0.100 g, 0.68 mmol). After the reaction mixture was stirred for 1 hour at room temperature under nitrogen protection, DDQ (0.232 g, 1.02 mmol) was added. After stirring for 2 hours, the mixture was diluted with ethyl acetate (100 mL). The organic layer was washed with sat. NaHCO₃ (3×20 mL) and brine (20 mL), and dried over Na₂SO₄. The solution was concentrated and purified by flash column chromatography using 15% EtOAc in hexane as eluent to afford 2,5-dichloro-3-(5-methoxy-1H-indole-3-yl)-[1,4]benzoquinone (0.211 g, 96%) as blue needles from benzene/hexane. Melting Point 195–196° C. ¹H NMR (CDCl₃): 6.78 (1H, d, J=2.4 Hz), 6.93 (1H, dd, J=2.1, 8.4 Hz), 7.23 (1H, s), 7.34 (1H, d, J=9.0 Hz), 7.54 (1H, d, J=3.0 Hz), 8.62 (1H, br s). ¹³C NMR (d₆-acetone): 55.2, 103.8, 106.7, 112.4, 112.8, 126.5, 130.9, 131.0, 131.4, 133.4, 139.8, 143.7, 154.8, 177.8, 178.0. IR (KBr): 3328, 3043, 2924, 2855, 1675, 1638, 1543, 1484, 1260, 1227 cm⁻¹. Anal. Calcd. for C₁₅H₉Cl₂NO₃: C, 55.93; H, 2.82; N, 4.35. Found: C, 55.72; H, 2.99; N, 4.24.

EXAMPLE 3 AcOH Preperation of 2,5-dichloro-3-[2-(1-methyl-cyclopropyl)-1H-indol-3-yl]-[1,4]benzoquinone (Procedure C)

To a solution of 2,5-dichloro-1,4-benzoquinone (0.177 g, 1.00 mmol) in AcOH (2 mL) was added 2-(1methyl-cyclopropyl)indole (0.081 g, 0.47 mmol). After the reaction mixture was stirred for 2 h at 50° C. under nitrogen protection, Ag₂CO₃ on Celite® (50%, 0.500 g, 0.90 mmol) was added. After stirring for 4 h at room temperature, the solution was diluted with ethyl acetate (100 mL). The organic layer was washed with sat. NaHCO₃ (3×20 mL) and brine (20 mL), and dried over Na₂SO₄. The solution was concentrated and purified by flash column chromatography using 15% EtOAc in hexane as eluent to afford 2,5-dichloro-3-[2-(1-methyl-cyclopropyl)-1H-indol-3-yl]-[1,4]benzoquinone (0.148 g, 90%) as blue needles from benzene/hexane. Melting Point 170–171° C. ¹H NMR (d₆-acetone): 0.53–0.60 (1H, m), 0.64–0.70 (1H, m), 0.70–0.77 (1H, m), 0.92–0.98 (1H, m), 1.48 (3H, s), 7.01 (1H, dt, J=1.2, 7.5 Hz), 7.10 (1H, dt, J=1.2, 7.5 Hz), 7.24 (1H, dd, J=0.6, 7.2 Hz), 7.37 (1H, d, J=7.8 Hz), 7.39 (1H, s), 10.70 (1H, s). ¹³C NMR (d₆-acetone): 13.3, 14.1, 15.1, 24.5, 103.6, 111.4, 119.7, 119.9, 121.9, 126.8, 133.8, 136.0, 140.4, 140.8, 143.9, 144.0, 177.1, 177.9. IR (KBr): 3409, 3059, 3004, 2966, 2927, 1662, 1620, 1567, 1435, 1270, 1241, 1028, 755 cm⁻¹. Anal. Calcd. for C₁₈H₁₃Cl₂NO₂: C, 62.45; H, 3.78; N, 4.05. Found: C, 62.30; H, 3.77; N, 3.94.

EXAMPLE 4 Hydrolysis of 2,5-dichloro-3-[2-(1-methyl-cyclopropyl)-1H-indol-3-yl]-[1,4]benzoquinone (Procedure D)

To a refluxing solution of 2,5-dichloro-3-[2-(1-methyl-cyclopropyl)-1H-indole-3-yl]-[1,4]benzoquinone (0.056 g, 0.16 mmol) in MeOH (6 mL) was added 10% aqueous NaOH (3 mL) dropwise. After refluxing for a half hour, the mixture was poured into cold water (20 mL). H₂SO₄ (10%) was added to acidify the mixture, which was extracted with EtOAc (3×10 mL). The organic layer was washed with brine (10 mL) and dried over Na₂SO₄. The solution was concentrated and purified by flash column chromatography (oxalic acid-coated silica gel) using 30% EtOAc in hexane as the eluent to afford 2,5-dihydroxy-3-[2-(1-methyl-cyclopropyl)-1H-indol-3-yl]-[1,4]benzoquinone (0.044 g, 87%) as dark blue needles from acetone/hexane. Melting Point 103° C. (dec). ¹H NMR (d₆-acetone): 0.55–0.62 (2H, m), 0.81–0.87 (2H, m), 1.45 (3H, s), 6.08 (1H, s), 6.93 (1H, dt, J=0.9, 8.1 Hz), 7.04 (1H, dt, J=1.2, 8.1 Hz), 7.22 (1H, d, J=7.8 Hz), 7.31 (1H, dd, J=0.9, 8.1 Hz), 9.0–10.1 (2H, br s), 10.34 (1H, br s). ¹³C NMR (d₆-acetone): 13.3, 14.8, 24.4, 101.4, 103.6, 110.8, 112.3, 119.0, 119.5, 121.1, 128.4, 135.8, 142.2. IR (KBr): 3300, 3077, 2958, 2924, 1640, 1360, 1190, 746 cm⁻¹. HRMS (EI): calculated for C₁₈H₁₅NO₄ 309.1001; found 309.0999.

EXAMPLE 5

The following indoles were reacted according to the above procedures (procedures A–C, Examples 1–3) to achieve the listed yields and stated product.

TABLE 1 Procedure Procedure Procedure Indole A^(a) B^(b) C^(b) End Product indole 70% 75% 8 2,5-Dichloro-3-(1H-indol-3-yl)- [1,4]benzoquinone 2-methylindole 97% 96% 2 2,5-Dichloro-3-(2-metthyl-1H-indol-3-yl)- [1,4]benzoquinone 2,5-dimethylindole 91% 2,5-Dichloro-3-(2,5dimethyl-1H-indol-3- yl)-[1,4]benzoquinone 2-methyl-5-methoxyindole^(c) 89% 2,5-Dichloro-3-(5-methoxy-2-methyl-1H- indol-3-yl)-[1,4]benzoquinone 2-methyl-5-chloroindole^(c) 75% 2,5-Dichloro-3-(5-chloro-2-methyl-1H- indol-3-yl)-[1,4]benzoquinone 2-cyclopropylindole 95% 2,5-Dichloro-3-(2-cyclopropyl-1H-indol-3- yl)-[1,4]benzoquinone 2-phenylindole 89% 2,5-Dichloro-3-(2-phenyl-1H-indol-3-yl)- [1,4]benzoquinone N-methylindole 87% 2,5-Dichloro-3-(1-methyl-1H-indol-3-yl)- [1,4]benzoquinone 2-ethylindole 95% 2,5-Dichloro-3-(2-ethyl-1H-indol-3-yl)- [1,4]benzoquinone 2-isopropylindole 70% 2,5-Dichloro-3-(2-isopropyl-1H-indol-3- yl)-[1,4]benzoquinone 2-tert-butylindole 54% 90% 2 2,5-Dichloro-3-(2-tert-butyl-1H-indol-3- yl)-[1,4]benzoquinone 2-(1-methylcyclopropyl)indole 46% 1 90% 2 2,5-Dichloro-3-[2-(1-methyl-cyclopropyl)- 1H-indol-3-yl]-[1,4]benzoquinone 2-(1-methylcyclohexyl)indole 50% 24 91% 10 2,5-Dichloro-3-[2-(1-methyl-cyclohexyl)- 1H-indol-3-yl]-[1,4]benzoquinone 4-fluoroindole  0% 120 16% 42 14% 22 3-(4-Fluoro-1H-indol-3-yl)-2,5-dichloro- [1,4]benzoquinone 4-chloroindole  0% 120 54% 92 3-(4-Chloro-1H-indol-3-yl)-2,5-dichloro- [1,4]benzoquinone 4-bromoindole  0% 120 39% 144 3-(4-Bromo-1H-indol-3-yl)-2,5-dichloro- [1,4]benzoquinone 4-methoxyindole  2% 0.5 66% 3 2,5-Dichloro-3-(4-methoxy-1H-indol-3-yl)- [1,4]benzoquinone 4-benzyloxyindole  1% 0.5 65% 3 3-(4-Benzyloxy-1H-indol-3-yl)-2,5- dichloro-[1,4]benzoquinone 4-methylindole 39% 21 76% 20 2,5-Dichloro-3-(4-methyl-1H-indol-3-yl)- [1,4]benzoquinone 5-fluoroindole 50% 5 74% 6 3-(5-Fluoro-1H-indol-3-yl)-2,5-dichloro- [1,4]benzoquinone 5-chloroindole 56% 0.5 72% 24 3-(5-Chloro-1H-indol-3-yl)-2,5-dichloro- [1,4]benzoquinone 5-bromoindole 57% 0.5 73% 24 3-(5-Bromo-1H-indol-3-yl)-2,5-dichloro- [1,4]benzoquinone 5-hydroxyindole 52% 0.5  6% 0.5 2,5-Dichloro-3-(5-hydroxy-1H-indol-3-yl)- [1,4]benzoquinone 5-methoxyindole 96% 0.5 2,5-Dichloro-3-(5-methoxy-1H-indol-3-yl)- [1,4]benzoquinone 5-benzyloxyindole 92% 0.5 3-(5-Benzyloxy-1H-indol-3-yl)-2,5- dichloro-[1,4]benzoquinone 5-methylindole 82% 6 72% 2 2,5-Dichloro-3-(5-methyl-1H-indol-3-yl)- [1,4]benzoquinone 6-fluoroindole 37% 5 63% 6 3-(6-Fluoro-1H-indol-3-yl)-2,5-dichloro- [1,4]benzoquinone 6-chloroindole 58% 20 55% 6 3-(6-Chloro-1H-indol-3-yl)-2,5-dichloro- [1,4]benzoquinone 6-benzyloxyindole 14% 2 22% 22 3-(6-Bromo-1H-indol-3-yl)-2,5-dichloro- [1,4]benzoquinone 6-methylindole 63% 1 75% 20 2,5-Dichloro-3-(6-methyl-1H-indol-3-yl)- [1,4]benzoquinone 7-methylindole 81% 83% 22 2,5-Dichloro-3-(7-propyl-1H-indol-3-yl)- [1,4]benzoquinone 7-propylindole 82% 6 81% 5 2,5-Dichloro-3-(7-methyl-1H-indol-3-yl)- [1,4]benzoquinone 7-tert-butylindole 81% 6 82% 8 3-(7-tert-butyl-1H-indol-3-yl)-2,5-dichloro- [1,4]benzoquinone 7-phenylindole 66% 12 66% 4 2,5-Dichloro-3-(7-phenyl-1H-indol-3-yl)- [1,4]benzoquinone 7-methoxyindole 13% 20 62% 9 50% 68 2,5-Dichloro-3-(7-methoxy-1H-indol-3-yl)- [1,4]benzoquinone 7-benzyloxyindole 71% 5 65% 6 3-(7-Benzyloxy-1H-indol-3-yl)-2,5- dichloro-[1,4]benzoquinone 7-fluoroindole 43   48 3-(7-Fluoro-1H-indol-3-yl)-2,5-dichloro- [1,4]benzoquinone 7-chloroindole 17   96 63   72 3-(7-Chloro-1H-indol-3-yl)-2,5-dichloro- [1,4]benzoquinone 7-bromoindole 34   72 41   48 3-(7-Bromo-1H-indol-3-yl)-2,5-dichloro- [1,4]benzoquinone 7-benzylindole 89   1 3-(7-Benzyl-1H-indol-3-yl)-2,5-dichloro- [1,4]benzoquinone 7-o-methylbenzylindole 88   1 2,5-Dichloro-3-[7-(2-methyl-benzyl)-1H- indol-3-yl]-[1,4]benzoquinone 1H-Benzo[g]indole 34% 48 3-(1H-Benzo[g]indol-3-yl)-2,5-dichloro- [1,4]benzoquinone 2,6-dimethylindole 89% 6 3-(2,6-Dimethyl-1H-indol-3-yl)-2,5- dichloro-[1,4]benzoquinone 2,7-dimethylindole 59% 2 94% 1 3-(2,7-Dimethyl-1H-indol-3-yl)-2,5- dichloro-[1,4]benzoquinone 6,7-dimethylindole 90% 0.16 88% 4 3-(6,7-Dimethyl-1H-indol-3-yl)-2,5- dichloro-[1,4]benzoquinone 5,6-methylenedioxyindole 10% 2 10% 2 3-(5,6-Methylenedioxy-1H-indol-3-yl)-2,5- dichloro-[1,4]benzoquinone 5,6-dimethoxyindole  9% 24 33% 0.5 3-(5,6-Dimethoxy-1H-indol-3-yl)-2,5- dichloro-[1,4]benzoquinone ^(a)variation from the standard reaction time (10 h) is given in the right column. ^(b)time for consumption of indole using THF as solvent is given in the right column. ^(c)used 0.3 eq HCl.

EXAMPLE 6

The mono-indolquinone starting products listed in Table 2 were reacted according procedure D (Example 4) to achieve the listed yields and stated end product. Also included in this example are characterizations of the starting and ending compounds.

TABLE 2 Starting Product Yield End Product 2,5-Dichloro-3-(1H-indol-3-yl)-[1,4]benzoquinone 59% 2,5-Dihydroxy-3-(1H-indol-3-yl)-[1,4]benzoquinone mp 124–125° C. IR(KBr): 3362, 3062, 2922, 1666, 1645, mp 219–220° C. IR(KBr): 3421, 3301, 1666, 1293, 1559, 1248cm⁻¹. ¹H NMR(CDCl₃): δ 7.19(1H, s), 7.20–7.28 1235, 931cm⁻¹. ¹H NMR(d₆-acetone): δ 10.6(1H, br (2H, m), 7.38(1H, m), 7.41(1H, m), 7.47(1H, d, J=3Hz), s), 9.0–10.0(2H, br), 7.59(1H, d, J=2.4Hz), 7.52 8.94(1H, br s). ¹³C NMR(CDCl₃): δ 107.2, 112.0, (1H, d, J=8.1Hz), 7.44(1H, d, J=8.1Hz), 7.11(1H, 121.3, 122.0, 123.3, 125.3, 129.8, 133.4, 135.8, 137.4, t, J=8.1Hz), 7.02(1H, t, J=8.1Hz), 6.01(1H, s); 138.5, 144.3, 177.5, 178.0. HRMS (EI) Calcd. For 13_(C) NMR(d₆-acetone): δ 136.4, 127.6, 126.9, 121.9, C₁₄H₇Cl₂NO₂ [M⁺]: 290.9854. Found: 290.9865. 121.5, 119.2, 112.1, 111.5, 104.7, 103.2. HRMS (FAB) Calculated for C₁₄H₉NO₄ [M⁺]: 255.0532. Found: 255.0529. 2,5-Dichloro-3-(2-methyl-1H-indol-3-yl)-[1,4]benzoquinone 62% 2,5-Dihydroxy-3-(2-metthyl-1H-indol-3-yl)- The residue was concentrated and purified by flash column [1,4]benzoquinone mp 222–223° C. IR(KBr): 3392, chromatography using 15% EtOAc in hexane as eluent to 3313, 1630, 1359, 1294, 1186cm⁻¹. ¹H NMR(d₆- afford pure 2,5-dichloro-3-(2-methyl-1H-indol-3-yl)- acetone): δ 10.27(1H, br s), 7.30(1H, d, J=8.1Hz), [1,4]benzoquinone (1.13 g, 97%)as a blue solid. mp 158–159° C. 7.23(1H, d, J=8.1Hz), 7.02(1H, t, J=8.1Hz), R_(f) =0.35(3:7, EtOAc/hexane). ¹H NMR(CDCl₃)δ 6.94(1H, t, J=8.1Hz), 6.04(1H, s), 2.32(3H, s). 8.40(bs, NH), 7.24–7.11(m, 5H), 2.26(s, 3H). ¹H NMR ¹³C NMR(d₆-acetone): δ 136.0, 135.5, 128.6, 120.6, (d₆-acetone): δ 10.60(1H, br s), 7.39(1H, s), 7.36(1H, d, J=7.8Hz), 119.8, 119.0, 111.9, 110.5, 103.4, 102.2, 12.8. 7.24(1H, d, J=7.8Hz), 7.10(1H, t, J=7.8Hz), HRMS (FAB) Calcd. For C₁₅H₁₁NO₄ [M⁺]: 7.01(1H, t, J=7.8Hz). ¹³C NMR(CDCl₃)δ 177.7, 176.6, 269.0688. Found: 269.0691. 144.3, 139.8, 139.3, 136.9, 135.1, 133.1, 126.6, 122.0, 120.5, 119.6, 110.8, 104.8, 13.9. IR(KBr): 3388, 3061, 1676, 1566, 1459, 1250, 1032, 749cm⁻¹. Anal. Calcd. for C₁₅H₉Cl₂NO₂: C, 58.85; H, 2.96; Cl, 23.16; N, 4.58. Found: C, 58.85; H, 2.96; Cl, 23.16; N, 4.58. HRMS (EI) Calcd. For C₁₅H₉Cl₂NO₂ [M⁺]: 305.0010. Found: 305.0002. 2,5-Dichloro-3-(2,5-dimethyl-1H-indol-3-yl)- 73% 2,5-Dihydroxy-3-(2,5dimethyl-1H-indol-3-yl)- [1,4]benzoquinone [1,4]benzoquinone Blue crystals from benzene/hexane. mp 185–186° C. IR Green crystals from benzene/hexane. mp 231–232° C. (KBr): 3346, 2922, 1671, 1656, 1559, 1259cm⁻¹. ¹H NMR IR(KBr): 3416, 3287, 2917, 2858, 1619, 1355, (CDCl₃): δ 2.32(3H, s), 2.41(3H, s), 6.94(1H, m), 6.97 1184cm⁻¹, ¹H NMR(d₆-acetone): δ 10.12(1H, br s), (1H, m), 7.18(1H, dd, J=0.6, 8.4Hz), 7.21(1H, s), 8.19 9.0–10.0(2H, br), 7.18(1H, d, J=8.1Hz), 7.01(1H, (1H, br s). ¹³C NMR(CDCl₃): δ 14.3, 21.9, 104.8, 110.7, s), 6.85(1H, d, J=8.4Hz), 6.04(1H, s), 2.33(3H, 119.8, 123.9, 127.2, 130.3, 133.4, 133.7, 137.1, 139.8, s), 2.29(3H, s). ¹³C NMR(d₆-acetone): δ 135.5, 140.1, 144.7, 177.0, 178.1. Anal. Calcd. for C₁₆H₁₁Cl₂NO₂: 134.4, 128.8, 127.6, 122.1, 119.6, 110.2, 103.4, C, 60.02; H, 3.46; N, 4.37. Found: C, 59.80; H, 3.58; N, 101.7, 21.1, 12.8, HRMS (FAB) Calcd. For 4.43. C₁₆H₁₃NO₄ [M⁺]: 283.0845. Found: 283.0846. 2,5-Dichloro-3-(5-methoxy-2-methyl-1H-indol-3-yl)- 81% 2,5-Dihydroxy-3-(5-methoxy-2-methyl-1H-indol-3- [1,4]benzoquinone yl)-[1,4]benzoquinone Blue crystals from benzene/hexane. mp 199.2–199.5° C. IR mp 212° C. (dec). IR (KBr): 3389, 3080, 2925, 1612, (KBr): 3357, 2938, 2895, 2820, 1672, 1645, 1565, 1275, 1484, 1358, 1329, 1198, 799cm⁻¹. ¹H NMR(d₆- 1253cm⁻¹. ¹H NMR(CDCl₃): δ 2.28(3H, s), 3.78(3H, s), acetone): δ 10.10(1H, br s), 9.0–9.8(2H, br), 7.16 6.58(1H, d, J=2.4Hz), 6.80(1H, dd, J=2.4, 9Hz), 7.17 (1H, d, J=6.6Hz), 6.75(1H, s), 6.65(1H, J=6.6Hz), (1H, d, J=9Hz), 7.22(1H, s), 8.22(1H, br s). ¹³C NMR 6.00(1H, s), 3.70(3H, s), 2.26(3H, s). ¹³C (CDCl₃): δ 14.4, 56.2, 102.5, 105.1, 111.8, 112.2, 127.5, NMR(d₆-acetone): δ 154.1, 136.2, 131.0, 129.1, 130.4, 133.4, 137.8, 139.7, 139.9, 144.7, 154.9, 176.9, 112.0, 111.0, 110.4, 103.3, 102.3, 102.1, 55.1, 12.7. 178.1. HRMS (EI) Calcd. For C₁₆H₁₁Cl₂NO₂ [M⁺]: HRMS (FAB) Calculated for C₁₆H₁₃NO₅ [M⁺]: 335.0116. Found: 335.0123. 299.0794. Found: 299.0795. 2,5-Dichloro-3-(5-chloro-2-methyl-1H-indol-3-yl)- 87% 2,5-Dihydroxy-3-(5-chloro-2-methyl-1H-indol-3-yl)- [1,4]benzoquinone [1,4]benzoquinone Blue crystals from benzene/hexane. mp 254–255° C. IR mp 230° C. (dec). IR(KBr): 3440, 3315, 1632, 1470, (KBr): 3336, 3078, 1678, 1656, 1570, 1275cm⁻¹. ¹H NMR 1358, 1184, 795cm⁻¹. ¹H NMR(d₆-acetone): δ 10.43 (d₆-acetone): δ 2.36(3H, s), 7.07(1H, dd, J=2.1, 8.7Hz), (1H, br s), 9.0–10.0(2H, br), 7.28(1H, d, J=6.3Hz), 7.30(1H, d, J=2.1Hz), 7.38(1H, dd, J=0.6, 8.7Hz), 7.39 7.23(1H, d, J=1.5Hz), 6.98(1H, dd, J=6.3, (1H, s), 10.9(1H, br s). ¹³C NMR(d₆-acetone): δ 13.0, 1.5Hz), 6.00(1H, s), 2.29(3H, s). ¹³C NMR(d₆- 104.7, 112.4, 119.1, 121.4, 125.1, 128.7, 133.4, 134.5, acetone): δ 137.5, 134.5, 129.7, 124.3, 120.5, 119.2, 139.1, 139.3, 140.5, 144.2, 176.9, 178.1. Calculated for 111.9, 111.0, 103.4, 12.6. HRMS (FAB) Calculated C₁₅H₈Cl₃NO₂: C, 52.90; H, 2.37; N, 4.11; Found: C, 52.79; for C₁₇H₁₃NO₄ [M⁺]: 303.0298. Found: 303.0302. H, 2.44; N, 3.97. 2,5-Dichloro-3-(2-cyclopropyl-1H-indol-3-yl)- 81% 2,5-Dihydroxy-3-(2-cyclopropyl-1H-indol-3-yl)- [1,4]benzoquinone [1,4]benzoquinone This compound was synthesized according to the general mp 179–180° C. IR(KBr): 3358, 2921, 1632, 1461, procedure A from 2-cyclopropyl-1H-indole (100 mg, 0.636 mmol) 1364, 1187, 955, 741cm⁻¹. ¹H NMR(d₆-acetone): δ and 2,5-dichloro-1,4-benzoquinone (225 mg, 1.27 mmol) 10.0(1H, br s), 9.2–10.0(2H, br), 7.27(1H, d, J=7.8Hz), and obtained as a blue solid (200 mg, 95%). mp 181–182° C. 7.21(1H, d, J=7.8Hz), 7.01(1H, t, J=7.8Hz), R_(f) =0.37(3:7, EtOAc/hexane). ¹H NMR(CDCl₃): δ 6.93(1H, t, J=7.8Hz), 6.05(1H, s), 1.99 8.32(1H, br s), 7.09–7.23(5H, m), 1.87(1H, m), 0.93(2H, (1H, m), 0.89(4H, m). ¹³C NMR(d₆-acetone): δ m), 0.70(2H, m). ¹³C NMR(CDCl₃)δ 177.8, 176.4, 144.3, 140.5, 135.7, 128.6, 120.7, 119.4, 119.1, 111.9, 141.9, 139.6(2C), 134.7, 133.0, 126.6, 122.1, 120.5, 119.5, 110.7, 103.5, 102.6, 9.2, 7.1. HRMS (FAB) 110.9, 105.3, 9.4, 7.3. IR(KBr): 3450, 3386, 3061, 1676, Calculated for C₁₇H₁₃NO₄ [M⁺]: 295.0845. Found: 1650, 1561, 1448, 1273, 1241, 1032, 882, 745cm⁻¹. Anal. 295.0846. Calcd. for C₁₇H₁₁Cl₂NO₂ : C, 61.47; H, 3.34; N, 4.22; Found: C, 61.71; H, 3.36; N, 4.20. 2,5-Dichloro-3-(2-phenyl-1H-indol-3-yl)-[1,4]benzoquinone 65% 2,5-Dihydroxy-3-(2-phenyl-1H-indol-3-yl)- Blue crystals from benzene/hexane. mp 170–171° C. ¹H [1,4]benzoquinone NMR(CDCl₃): δ 7.20(1H, m), 7.21(1H, s), 7.28–7.31(2H, Green crystals from benzene/hexane. mp 227–228° C. m), 7.36–7.43(5H, m), 7.47(1H, m), 8.61(1H, br s). ¹³C ¹H NMR(d₆-acetone): δ 10.8(1H, br s), 9.0–10.0 NMR(CDCl₃): δ 104.7, 111.9, 120.4, 121.3, 123.3, 127.3, (2H, br), 7.67(2H, m), 7.45(1H, m), 7.38(3H, m), 127.5, 129.1, 129.4, 132.4, 133.4, 136.2, 139.6, 140.5, 7.29(1H, m), 7.12(1H, t, J=8.4Hz), 7.03(1H, t, J=7.2Hz), 141.3, 144.7, 176.5, 177.8. IR(KBr): 3384, 3056, 1683, 6.06(1H, s). ¹³C NMR(d₆-acetone): δ 1565, 1446, 1248cm⁻¹. Anal. Calcd. for C₂₀H₁₁Cl₂NO₂: C, 137.0, 136.7, 133.7, 129.1, 128.8, 127.7, 127.2, 65.24; H, 3.01; N, 3.73. Found: C, 64.61; H, 3.14; N, 3.76. 122.1, 120.3, 119.6, 112.1, 111.4, 103.8, 102.3. IR (KBr): 3422, 3303, 3051, 1629, 1355, 1296cm⁻¹. HRMS (FAB) Calcd. For C₂₀H₁₃NO₄ [M⁺]: 331.0845. Found: 331.0846. 2,5-Dichloro-3-(1-methyl-1H-indol-3-yl)-[1,4]benzoquinone 62% 2,5-Dihydroxy-3-(1-methyl-1H-indol-3-yl)- Blue crystals from benzene/hexane. mp 156.1–156.2° C. IR [1,4]benzoquinone (KBr): 3142, 3057, 2911, 1683, 1656, 1559, 749cm⁻¹. ¹H Green crystals from benzene/hexane. mp 201–202° C. NMR(CDCl₃): δ 3.91(3H, s), 7.21(1H, s), 7.23(1H, m), IR(KBr): 3286, 1627, 1533, 1355, 1298, 1234, 7.31(1H, m), 7.39(1H, d, J=9.3Hz), 7.40(1H, dd, J=0.9, 1188, 935, 735cm⁻¹. ¹H NMR(d₆-acetone): δ 9.0–10.0 6.9Hz). ¹³C NMR(CDCl₃): δ 33.9, 105.6, 110.3, 121.1, (2H, br)7.52(1H, d, J=8.1Hz), 7.49(1H, s), 122.3, 122.9, 126.0, 133.5, 134.4, 137.0, 138.4, 144.1, 7.41(1H, d, J=8.4Hz), 7.19(1H, t, J=7.5Hz), 178.0. Anal. Calcd. for C₁₅H₉Cl₂NO₂: C, 58.85; H, 2.97; N, 7.05(1H, t, J=7.5Hz),, 6.01(1H, s), 3.89(3H, s). 4.58. Found: C, 58.69; H, 3.07; N, 4.57. ¹³C NMR(d₆-acetone): δ 137.0, 131.6, 127.4, 122.2, 121.5, 119.2, 111.8, 109.5, 103.6, 103.2, 32.4. HRMS (FAB) Calculated for C₁₅H₁₁NO₄ [M⁺]: 269.0688. Found: 269.0681. 2,5-Dichloro-3-(2-ethyl-1H-indol-3-yl)-[1,4]benzoquinone 77% 2,5-Dihydroxy-3-(2-ethyl-1H-indol-3-yl)- This compound was synthesized according to the general [1,4]benzoquinone procedure A from 2-ethyl-1H-indole(100 mg, 0.689 mmol) Green crystals from benzene/hexane. mp 157–158° C. and 2,5-dichloro-1,4-benzoquinone(244 mg, 1.38 mmol) IR(KBr): 3395, 3320, 3062, 2976, 1629, 1361, and was obtained as a blue solid(209 mg, 95%). mp 143–144° C. 1291, 1173cm⁻¹. ¹H NMR(d₆-acetone): δ 10.27(1H, R_(f) =0.38(3:7, EtOAc/hexane). ¹H NMR(CDCl₃): δ br s), 9.0–10.0(2H, br), 7.31(1H, d, J=7.8Hz), 7.22 8.48(bs, NH), 7.29(m, 1H), 7.20(s, 1H), 7.19–7.08(m, (1H, d, J=7.8Hz), 6.94–7.08(2H, m), 6.04(1H, s), 3H), 2.61(qd, J=7.5, 3.0Hz, 2H), 1.27(t, J=7.5Hz, 3H); 2.68(2H, q, J=7.8Hz), 1.28(3H, t, J=6.9Hz). ¹³C ¹H NMR(d₆-acetone): δ 10.62(1H, br s), 7.40(1H, s), 7.38 NMR(d₆-acetone): δ 140.8, 136.2, 128.4, 120.7, (1H, d, J=8.1Hz), 7.24(1H, d, J=8.1Hz), 7.09(1H, t, J=8.1Hz), 119.8, 119.0, 111.8, 110.7, 103.5, 101.1, 20.9, 13.4. 8.1Hz), 7.01(1H, t, J=8.1Hz), 7.71(2H, q, J=7.8Hz), HRMS (FAB) Calcd. For C₁₆H₁₃NO₄ [M⁺]: 1.30(3H, t, J=7.8). ¹³C NMR(CDCl₃): δ 177.7, 176.6, 283.0845. Found: 283.0846. 144.3, 142.1, 140.2, 139.5, 135.2, 133.0, 126.4, 121.9, 120.5, 119.6, 110.9, 103.7, 21.2, 13.4. IR(KBr): 3394, 3050, 2973, 1677, 1646, 1563, 1447, 1241, 1038, 746cm⁻¹. Anal. Calcd. for C₁₆H₁₁Cl₂NO₂: C, 60.02; H, 3.46; Cl, 22.15; N, 4.37. Found. C, 59.99; H, 3.51; N, 4.18. 2,5-Dichloro-3-(2-isopropyl-1H-indol-3-yl)- 80% 2,5-Dihydroxy-3-(2-isopropyl-1H-indol-3-yl)- [1,4]benzoquinone [1,4]benzoquinone This compound was synthesized according to the general mp 140° C.(dec). IR(KBr): 3352, 3290, 2926, 1627, procedure A from 2-isoprenyl-1H-indole(50 mg, 0.314 mmol) 1363, 1306, 1189, 955, 744cm⁻¹. ¹H NMR(d₆- and 2,5-dichloro-1,4-benzoquinone(111 mg, 0.629 mmol) acetone): δ 10.29(1H, br s), 9.0–10.0(2H, br), 7.31 and was obtained as a blue solid(74 mg, 70%). mp (1H, d, J=7.8Hz), 7.21(1H, d, J=7.8Hz), 7.02(1H, 187–188° C. R_(f) =0.26(1:4, EtOAc/hexane). ¹H NMR t, J=7.8Hz), 6.93(1H, t, J=7.8Hz), 6.05(1H, s), (CDCl₃)δ 8.40(bs, NH), 7.36(m, 1H), 7.22(s, 1H), 7.21–7.08 3.03(1H, m), 1.32(6H, d, J=6.9Hz). ¹³C NMR(d₆- 7.08(m, 3H), 2.88(septet, J=6.9Hz, 1H), 1.35(d, J=6.9Hz, acetone): δ 144.7, 136.3, 128.3, 120.7, 119.7, 118.9, 3H), 1.27(d, J=6.9Hz, 3H). ¹³C NMR(CDCl₃)δ 111.9, 110.8, 103.5, 100.1, 27.3, 22.2. HRMS (FAB) 177.7, 176.6, 145.8, 144.4, 140.9, 139.7, 135.1, 133.1, Calculated for C₁₇H₁₅NO₄ [M⁺]: 297.1001. Found: 126.1, 122.0, 120.4, 119.5, 111.0, 102.6, 27.4, 22.9, 22.3. IR 297.0997. (KBr): 3421, 3312, 3053, 2963, 1671, 1569, 1440, 1241, 1031, 759cm⁻¹. HRMS (EI) Calcd. For C₁₇H₁₃Cl₂NO₂ [M⁺]: 333.0323. Found: 333.0313 2,5-Dichloro-3-(2-tert-butyl-1H-indol-3-yl)- 52% 2,5-Dihydroxy-3-(2-tert-butyl-1H-indol-3-yl)- [1,4]benzoquinone [1,4]benzoquinone mp 235–236° C. IR(KBr)3419, 3317, 3059, 2965, 1673, mp 195° C.(dec). IR(KBr): 3434, 3329, 2963, 1634, 1585, 1462, 1245, 1029, 742cm⁻¹. ¹H NMR(d6-acetone): δ 1597, 1365, 1239, 944, 745cm⁻¹. ¹H NMR(d6- 10.40(1H, br s), 7.47(1H, s), 7.35(1H, d, J=7.8Hz), acetone): δ 10.13(1H, br s), 8.8–9.8(2H, br), 7.27 7.23(1H, d, J=7.8Hz), 7.08(1H, t, J=7.8Hz), 6.95(1H, t, (1H, d, J=6.0Hz), 7.14(1H, d, J=6.0Hz), 6.99(1H, J=7.8Hz), 1.38(9H, s). ¹³C NMR(CDCl₃): δ 178.0, 177.7, t, J=6.0Hz), 6.88(1H, t, J=6.0Hz), 6.05(1H, s), 145.3, 145.1, 143.2, 142.7, 134.9, 133.5, 127.1, 122.6, 1.34(9H, s). ¹³C NMR(d₆-acetone): δ 145.2, 135.6, 120.6, 118.3, 111.1, 102.1, 33.6, 30.5. HRMS (EI) Calcd. 129.3, 121.0, 118.9, 118.5, 113.8, 110.7, 103.8, 99.5, For C₁₈H₁₅Cl₂NO₂ [M⁺]: 347.0480. Found: 347.0491. 33.3, 29.7. HRMS (FAB) Calculated for C₁₇H₁₃NO₄ [M⁺]: 311.1158. Found: 311.1158 2,5-Dichloro-3-[2-(1-methyl-cyclopropyl)-1H-indol-3-yl]- 87% 2,5-Dihydroxy-3-[2-(1-methyl-cyclopropyl)-1H- [1,4]benzoquinone indol-3-yl]-[1,4]benzoquinone Blue needles from benzene/hexane. mp 170–171° C. ¹H The solution was concentrated and purified by flash NMR(d₆-acetone): 0.53–0.60(1H, m), 0.64–0.70(1H, m), column chromatography(oxalic acid-coated silica 0.70–0.77(1H, m), 0.92–0.98(1H, m), 1.48(3H, s), 7.01 gel)using 30% EtOAc in hexane as the eluent to (1H, dt, J=1.2, 7.5Hz), 7.10(1H, dt, J=1.2, 7.5Hz), 7.24 afford 2,5-dihydroxy-3-[2-(1-methyl-cyclopropyl)- (1H, dd, J=0.6, 7.2Hz), 7.37(1H, d, J=7.8Hz), 7.39(1H, 1H-indol-3-yl]-[1,4]benzoquinone(0.044 g, 87%)as s), 10.70(1H, s). ¹³C NMR(d₆-acetone): 13.3, 14.1, 15.1, dark blue needles from acetone/hexane. mp 103° C. 24.5, 103.6, 111.4, 119.7, 119.9, 121.9, 126.8, 133.8, 136.0, (dec). ¹H NMR(d₆-acetone): 0.55–0.62(2H, m), 140.4, 140.8, 143.9, 144.0, 177.1, 177.9. IR(KBr): 3409, 0.81–0.87(2H, m), 1.45(3H, s), 6.08(1H, s), 6.93 3059, 3004, 2966, 2927, 1662, 1620, 1567, 1435, 1270, (1H, dt, J=0.9, 8.1Hz), 7.04(1H, dt, J=1.2, 8.1Hz), 1241, 1028, 755cm⁻¹. Anal. Calcd. for C₁₈H₁₃Cl₂NO₂: C, 7.22(1H, d, J=7.8Hz), 7.31(1H, dd, J=0.9, 62.45; H, 3.78; N, 4.05. Found: C, 62.30; H, 3.77; N, 3.94. 8.1Hz), 9.0–10.1(2H, br s), 10.34(1H, br s). ¹³C NMR(d₆-acetone): 13.3, 14.8, 24.4, 101.4, 103.6, 110.8, 112.3, 119.0, 119.5, 121.1, 128.4, 135.8, 142.2. IR(KBr): 3300, 3077, 2958, 2924, 1640, 1360, 1190, 746cm⁻¹. HRMS (EI): calculated for C₁₈H₁₅NO₄ 309.1001; found 309.0999. 2,5-Dichloro-3-[2-(1-methyl-cyclohexyl)-1H-indol-3-yl]- 90% 2,5-Dihydroxy-3-[2-(1-methyl-cyclohexyl)-1H- [1,4]benzoquinone indol-3-yl]-[1,4]benzoquinone Blue needles from benzene/hexane. mp 184–185° C. ¹H Dark blue needles from acetone/hexane. mp 191–192° C. NMR(d₆-acetone): 1.36(3H, s), 1.38–1.66(10H, m), 6.96 ¹H NMR(d₆-acetone): 1.34(3H, s), 1.25–1.60 (1H, dt, J=1.2, 7.2Hz), 7.08(1H, dt, J=1.2, 7.2Hz), 7.23 (10H, m), 6.08(1H, s), 6.90(1H, dt, J=1.2, 6.9Hz), (1H, d, J=7.8Hz), 7.37(1H, d, J=8.1Hz), 7.45(1H, s), 7.02(1H, dt, J=1.2, 7.2Hz), 7.17(1H, d, J=7.8Hz), 10.39(1H, br s). ¹³C NMR(d₆-acetone): 22.6, 22.8, 26.2, 7.32(1H, dt, J=0.9, 8.1Hz), 8.5–10.0(2H, br 27.4, 37.5, 37.8, 38.2, 102.4, 111.2, 118.5, 119.5, 121.5, s), 10.12(1H, br s). ¹³C NMR(d₆-acetone): 23.0, 127.4, 134.0, 135.6, 142.4, 142.8, 143.9, 145.0, 177.8. IR 26.3, 37.6, 37.8, 100.4, 103.8, 110.7, 113.8, 118.5, (KBr): 3421, 3320, 3054, 2936, 2863, 1672, 1618, 1573, 118.8, 120.9, 129.4, 135.6, 143.8. IR(KBr): 3270, 1515, 1457, 1205, 1024cm⁻¹. Anal. Calcd. for 2975, 2932, 2862, 1700, 1535, 1465, 938, 717cm⁻¹. C₂₁H₁₉Cl₂NO₂: C, 64.96; H, 4.93; N, 3.61. Found: C, 65.04; HRMS (EI): calcd. for C₂₁H₂₁NO₄ 351.1471, found H, 4.89; N, 3.63. 351.1471. 3-(4-Fluoro-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone 65% 3-(4-Fluoro-1H-indol-3-yl)-2,5-dihydroxy- Blue needles from benzene/hexane. mp 161–162° C. ¹H [1,4]benzoquinone NMR(d₆-acetone): 6.79(1H, ddd, J=0.7, 7.6, 11.2Hz), Dark blue needles from acetone/hexane. mp 248° C. 7.15(1H, dt, J=5.2, 7.6Hz), 7.34(1H, d, J=8Hz), 7.38 (dec). ¹H NMR(d₆-acetone): 6.04(1H, s), 6.70(1H, (1H, s), 7.66(1H, d, J=2.8Hz), 11.15(1H, s). ¹³C NMR dd, J=7.8, 11.1Hz), 7.09(1H, dt, J=5.1, 8.1Hz), (d₆-acetone): 104.5, 105.7, 105.9, 108.6, 123.1, 123.2, 7.28(1H, dd, J=3.3, 8.1Hz), 7.43(1H, d, J=2.4Hz), 129.2, 133.6, 139.1, 143.6, 154.9, 157.2, 177.5 177.8. IR 8.0–10.4(2H, br s), 10.76(1H, br s). ¹³C NMR (KBr): 3386, 3067, 1671, 1577, 1505, 1422, 1318, 1265, (d₆-acetone): 103.5, 104.6, 104.9, 108.0, 122.2, 1226, 1019cm⁻¹. Anal. Calcd. for C₁₄H₆Cl₂FNO₂: C, 54.22; 122.3, 126.8, 139.4, 155.0, 158.3. IR(KBr): 3452, H, 1.95; N, 4.52. Found: C, 54.15; H, 1.89; N, 4.58. 2954, 2852, 1661, 1639, 1359, 1232, 1192, 1035, 934cm⁻¹. HRMS (EI): calcd. for C₁₄H₈FNO₄ 273.0437, found 273.0436. 3-(4-Chloro-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone 74% 3-(4-Chloro-1H-indol-3-yl)-2,5-dihydroxy- Blue needles from benzene/hexane. mp 204–205° C. ¹H [1,4]benzoquinone NMR(d₆-acetone): 7.07(1H, dd, J=0.8, 7.6Hz), 7.15(1H, Dark blue needles from acetone/hexane. mp 230° C. t, J=7.6Hz), 7.40(1H, s), 7.49(1H, dd, J=0.8, 8.0Hz), (dec). ¹H NMR(d₆-acetone): 6.05(1H, s), 7.01(1H, 7.60(1H, d, J=2.8Hz), 11.17(1H, br s). ¹³C NMR(d₆- dd, J=0.8, 7.6Hz), 7.10(1H, t, J=8.0Hz), 7.40 acetone): 106.3, 111.4, 121.0, 123.2, 124.4, 125.0, 128.4, (1H, d, J=2.4Hz), 7.44(1H, dd, J=0.8, 8.0Hz), 133.6, 137.9, 140.5, 140.9, 143.9, 177.7, 177.9. IR(KBr): 8.6–10.1(2H, br s), 10.77(1H, br s)¹³C NMR(d₆- 3350, 3115, 3068, 1673, 1659, 1572, 1416, 1274, 1196, acetone): 103.6, 104.0, 110.9, 112.5, 120.4, 122.3, 1023cm⁻¹. Anal. Calcd. for C₁₄H₆Cl₃NO₂: C, 51.49; H, 124.9, 125.6, 127.2, 138.1. IR(KBr): 3324, 2953, 1.85. Found: C, 51.33; H, 2.00. 1628, 1536, 1484, 1424, 1353, 1187, 927cm⁻¹. HRMS (FAB) : calcd. for C₁₄H₈ClNO₄ 289.0142, found 289.0143. 3-(4-Bromo-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone 86% 3-(4-Bromo-1H-indol-3-yl)-2,5-dihydroxy- Blue needles from benzene/hexane. mp 247–248° C. ¹H [1,4]benzoquinone NMR(d₆-acetone): 7.09(1H, t, J=8.0Hz), 7.25(1H, dd, J=0.8, Dark blue needles from acetone/hexane. mp 232° C. 8.0Hz), 7.41(1H, s), 7.54(1H, dd, J=0.8, 8.0Hz), (dec). ¹H NMR(d₆-acetone): 6.06(1H, s), 7.04(1H, 7.60(1H, d, J=3.2Hz), 11.11(1H, br s). ¹³C NMR(d₆- t, J=8.0Hz), 7.20(1H, dd, J=0.8, 8.0Hz), 7.41 acetone): 107.3, 111.2, 111.9, 113.1, 123.5, 124.3, 126.0, (1H, d, J=2.8Hz), 7.50(1H, dd, J=0.8, 8.0Hz), 128.2, 133.6, 137.7, 141.0, 144.1, 177.6, 177.9. IR(KBr): 9.0–10.0(2H, br s), 10.77(1H, br s). ¹³C NMR(d₆- 3367, 3116, 3068, 1651, 1613, 1570, 1517, 1480, 1412, acetone): 103.6, 104.9, 111.4, 113.7, 122.7, 123.7, 1021, 875cm⁻¹. Anal. Calcd. for C₁₄H₆BrCl₂NO₂: C, 45.32; 126.3, 127.2, 127.3, 137.8. IR(KBr). 3326, 2983, H, 1.63. Found: C, 45.38; H, 1.84. 2854, 1629, 1540, 1341, 1187, 934cm⁻¹. HRMS (EI): calcd. for C₁₄H₈BrNO₄ 332.9637, found 332.9634. 2,5-Dichloro-3-(4-methoxy-1H-indol-3-yl)- 82% 2,5-Dihydroxy-3-(4-methoxy-1H-indol-3-yl)- [1,4]benzoquinone [1,4]benzoquinone Blue needles from benzene/hexane. mp 195–196° C. ¹H Dark green needles from acetone/hexane. mp 225° C. NMR(d₆-acetone): 3.76(1H, s), 6.58(1H, dd, J=3.6, 5.1Hz), (dec). ¹H NMR(d₆-acetone): 3.71(3H, s), 6.00(1H, 7.11(1H, s), 7.12(1H, d, J=1.5Hz), 7.35(1H, s), 7.56 s), 6.48(1H, dd, J=3.2, 5.6Hz), 7.03(1H, d, J=2.0Hz), (1H, d, J=3Hz), 10.86(1H, br s). ¹³C NMR(d₆-acetone): 7.04(1H, s), 7.23(1H, d, J=2.8Hz), 8.5–9.8 55.0, 101.2, 105.4, 106.5, 123.6, 126.8, 127.0, 133.2, 136.4, (2H, br s), 10.42(1H, br s). ¹³C NMR(d₆-acetone): 138.0, 141.9, 143.6, 153.3, 177.6, 177.8. IR(KBr): 3324, 54.9, 100.2, 103.2, 103.7, 105.0, 113.9, 118.2, 122.6, 3042, 2967, 1676, 1638, 1546, 1486, 1260, 1229, 1009cm⁻¹. 124.6, 138.2, 154.3. IR(KBr): 3314, 2958, 2933, Anal. Calcd. for C₁₅H₉Cl₂NO₃: C, 55.93; H, 2.82; N, 4.35. 1634, 1511, 1357, 1200, 1089, 936cm⁻¹. HRMS Found: C, 56.09; H, 2.87; N, 4.32. (EI): calcd. for C₁₅H₁₁NO₅ 285.0637, found 285.0635. 3-(4-Benzyloxy-1H-indol-3-yl)-2,5-dichloro- 81% 3-(4-Benzyloxy-1H-indol-3-yl)-2,5-dihydroxy- [1,4]benzoquinone [1,4]benzoquinone Blue needles from benzene/hexane. mp 193–194° C. ¹H Dark green needles from acetone/hexane. mp 188–189° C. NMR(d₆-acetone): 5.01(2H, s), 6.64(1H, dd, J=2.4, 6.4Hz), ¹H NMR(d₆-acetone): 5.03(2H, s), 5.88 7.06(1H, s), 7.4–7.7(2H, m), 7.23–7.34(5H, m), 7.45 (1H, s), 6.60(1H, dd, J=1.6, 7.2Hz), 7.06(1H, q, J=8.4Hz), (1H, d, J=2.8Hz), 10.79(1H, br s). ¹³C NMR(d₆-acetone): 7.07(1H, d, J=2.8Hz), 7.21(1H, d, J=2.4Hz), 70.3, 102.1, 105.6, 106.3, 117.8, 123.5, 126.3, 127.8, 128.0, 7.24–7.32(3H, m), 7.34(1H, d, J=1.6Hz), 128.6, 132.9, 137.5, 138.1, 141.7, 143.8, 152.7, 177.2, 7.36(1H, d, J=2.0Hz), 8.8–9.8(2H, br s), 10.44 177.8. IR(KBr): 3346, 3116, 3063, 3034, 1673, 1656, 1572, (1H, br s). ¹³C NMR(d₆-acetone): 69.7, 101.1, 103.0, 1508, 1453, 1423, 1270cm⁻¹. Anal. Calcd. for 103.7, 105.2, 113.8, 118.3, 122.6, 124.5, 127.2, C₂₁H₁₃Cl₂NO₃: C, 63.34; H, 3.29. Found: C, 63.26; H, 3.40. 127.6, 128.4, 137.9, 138.3, 153.4. IR(KBr): 3396, 3308, 2918, 2869, 1628, 1506, 1443, 1318, 1075cm⁻¹. HRMS (EI): calcd. for C₂₁H₁₅NO₅ 361.0950, found 361.0952. 2,5-Dichloro-3-(4-methyl-1H-indol-3-yl)-[1,4]benzoquinone 81% 2,5-dihydroxy-3-(4-methyl-1H-indol-3-yl)- Blue needles from benzene/hexane. mp 192–193° C. ¹H [1,4]benzoquinone NMR(d₆-acetone): 2.28(3H, s), 6.83(1H, dt, J=0.6, 5.4Hz), Dark green needles from acetone/hexane. mp 220° C. 7.06(1H, t, J=5.7Hz), 7.32(1H, d, J=6Hz), 7.39 (dec). ¹H NMR(d₆-acetone): 2.36(3H, s), 6.05(1H, (1H, s), 7.44(1H, d, J=2.1Hz), 10.77(1H, br s). ¹³C NMR s), 6.76(1H, dt, J=0.9, 6.9Hz), 7.00(1H, t, J=7.6Hz), (d₆-acetone): 19.3, 107.1, 110.0, 122.0, 122.5, 126.1, 127.1, 7.23(1H, d, J=2.4Hz), 7.27(1H, d, J=8.1Hz), 129.8, 133.8, 136.9, 140.4, 142.0, 143.7, 177.9, 178.3. IR 8.5–10.2(2H, br s), 10.41(1H, br s). ¹³C NMR (KBr): 3369, 3114, 3068, 1669, 1553, 1511, 1267, 1182, (d₆-acetone): 18.7, 103.7, 104.4, 109.6, 114.1, 121.0, 1019, 876, 753cm⁻¹ . Anal. Calcd. for C₁₅H₉Cl₂NO₂: C, 121.7, 125.6, 126.8, 130.1, 136.9. IR(KBr): 3417, 58.85; H, 2.96. Found: C, 58.85; H, 2.91. 3328, 3128, 2959, 2927, 2861, 1628, 1533, 1357, 934cm⁻¹. HRMS (EI): calcd. for C₁₅H₁₁NO₄ 269.0688, found 269.0688. 3-(5-Fluoro-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone 72% 3-(5-Fluoro-1H-indol-3-yl)-2,5-dihydroxy- Blue needles from benzene/hexane. mp 175–176° C. ¹H [1,4]benzoquinone NMR(d₆-acetone): 7.00(1H, dt, J=2.1, 9.0Hz), 7.16(1H, Dark blue needles from acetone/hexane. mp 225–226° C. dd, J=1.2, 10.2Hz), 7.35(1H, s), 7.53(1H, dd, J=4.5, 8.7Hz), ¹H NMR(d₆-acetone): 6.03(1H, s), 6.93(1H, dt, 7.79(1H, d, J=2.7Hz), 11.1(1H, br s). ¹³C NMR(d₆- J=2.4, 9.2Hz), 7.26(1H, dd, J=2.4, 6.4Hz), 7.44 acetone): 106.4, 106.7, 110.3, 110.7, 113.2, 113.3, 132.2, (1H, dd, J=4.8, 8.8Hz), 7.69(1H, d, J=2.4Hz), 133.0, 133.3, 143.8, 156.5, 159.6, 177.4, 177.8. IR(KBr): 8.2–10.4(2H, br s), 10.69(1H, br s). ¹³C NMR(d₆- 3394, 3131, 3063, 2956, 2925, 1678, 1656, 1570, 1423, acetone): 103.2, 106.6, 106.9, 109.5, 109.8, 112.3, 1258, 1011cm⁻¹. Anal. Calcd. for C₁₄H₆Cl₂FNO₂: C, 54.22; 112.4, 129.6, 133.0, 156.5, 158.8. IR(KBr): 3449, H, 1.95; N, 4.52. Found: C, 54.30; H, 2.01; N, 4.54. 3294, 2956, 2855, 1627, 1486, 1335, 1230, 1184, 933cm⁻¹. HRMS (EI): calcd. for C₁₄H₈FNO₄ 273.0437, found 273.0442. 3-(5-Chloro-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone 62% 3-(5-Chloro-1H-indol-3-yl)-2,5-dihydroxy- Blue needles from benzene/hexane. mp 189–190° C. ¹H [1,4]benzoquinone NMR(CDCl₃): 7.24(1H, s), 7.25(1H, dd, J=0.9, 2.1Hz), Dark blue needles from acetone/hexane. mp 192–193° C. 7.36(1H, d, J=0.9Hz), 7.37–7.39(1H, m), 7.58(1H, d, J=3Hz), ¹H NMR(d₆-acetone): 6.03(1H, s), 7.12(1H, dd, 8.60(1H, s). ¹³C NMR(d₆-acetone): 106.4, 113.6, J=2.1, 8.7Hz), 7.46(1H, d, J=8.7Hz), 7.56(1H, d, 120.9, 122.4, 125.7, 127.2, 131.7, 133.4, 134.9, 137.5, J=1.8Hz), 7.67(1H, d, J=2.7Hz), 8.5–10.0(2H, 138.7, 143.9, 177.5, 177.9. IR(KBr): 3331, 3053, 1675, br s), 10.78(1H, br s). ¹³C NMR(d₆-acetone): 103.2, 1652, 1568, 1505, 1460, 1274, 1247, 803cm⁻¹. Anal. Calcd. 104.6, 111.3, 112.9, 121.3, 121.6, 124.6, 128.0, for C₁₄H₆Cl₃NO₂: C, 51.49; H, 1.85; N, 4.29. Found: C, 129.3, 134.8. IR(KBr): 3409, 3302, 2917, 2879, 51.51; H, 2.01; N, 4.26. 1631, 1529, 1354, 1231, 933cm⁻¹. HRMS (EI): calcd. for C₁₄H₈ClNO₄ 289.0142, found 289.0144. 3-(5-Bromo-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone 56% 3-(5-Bromo-1H-indol-3-yl)-2,5-dihydroxy- Blue needles from benzene/hexane. mp 205–206° C. ¹H [1,4]benzoquinone NMR(d₆-acetone): 7.31(1H, dd, J=2.0, 8.8Hz), 7.37(1H, Dark blue needles from acetone/hexane. mp 210–211° C. s), 7.50(1H, d, J=8.4Hz), 7.62(1H, s), 7.76(1H, d, J=2.8Hz), ¹H NMR(d₆-acetone): 6.02(1H, s), 7.24(1H, dd, 11.14(1H, s). ¹³C NMR(d₆-acetone): 106.3, 113.3, J=1.8, 8.7Hz), 7.42(1H, d, J=8.7Hz), 7.65(1H, 114.0, 123.9, 125.0, 127.8, 131.5, 133.4, 135.2, 137.5, d, J=2.7Hz), 7.71(1H, d, J=2.1Hz), 8.5–10.2(2H, 138.8, 143.9, 177.5, 177.9. IR(KBr): 3382, 3138, 3058, br s), 10.82(1H, br s). ¹³C NMR(d₆-acetone): 103.2, 1671, 1650, 1571, 1457, 1271, 1245, 1113, 1010cm⁻¹. Anal. 104.5, 112.2, 113.3, 124.2, 124.4, 128.6, 129.0, Calcd. for C₁₄H₆BrCl₂NO₂: C, 45.32; H, 1.63; N, 3.78. 129.1, 135.1 IR(KBr): 3309, 3035, 1631, 1455, Found: C, 45.56; H, 1.77; N, 3.70. 1349, 1322, 1230, 930cm⁻¹. HRMS (EI): calcd. for C₁₄H₈BrNO₄ 332.9637, found 332.9636. 2,5-Dichloro-3-(5-hydroxy-1H-indol-3-yl)- 70% 2,5-Dihydroxy-3-(5-hydroxy-1H-indol-3-yl)- [1,4]benzoquinone [1,4]benzoquinone Blue needles from benzene/hexane. mp 101(dec)° C. ¹H Dark green needles from acetone/hexane. mp 235° C. NMR(d₆-acetone): 6.80(2H, m), 7.33(1H, s), 7.34(1H, m), (dec). ¹H NMR(d₆-acetone): 6.00(1H, s), 6.73(1H, 7.65(1H, d, J=3.3Hz), 7.92(1H, br s), 10.86(1H, br s). dd, J=2.4, 8.7Hz), 6.92(1H, d, J=2.4Hz), 7.26 ¹³C NMR(d₆-acetone): 103.7, 105.9, 106.2, 112.4, 112.6, (1H, d, J=8.7Hz), 7.51(1H, d, J=3.0Hz), 7.6–7.8 113.4, 113.8, 131.1, 131.1, 131.3, 133.4. IR(KBr): 3500, (1H, br s), 8.0–10.0(2H, br s), 10.39(1H, br s). ¹³C 3121, 1698, 1566, 1167, 1102, 851, 694, 617cm⁻¹. Anal. NMR(d₆-acetone): 103.1, 104.0, 106.2, 111.7, 112.2, Calcd. for C₁₄H₇Cl₂NO₃: C, 54.57; H, 2.29. Found: C, 127.8, 128.0, 128.2, 131.1, 151.0 IR(KBr): 3302, 54.95; H, 2.62. 1697, 1630, 1463, 1357, 1202, 935, 856, 802cm⁻¹. HRMS (EI): calcd. for C₁₄H₉NO₅ 271.0481, found 271.0482. 2,5-Dichloro-3-(5-methoxy-1H-indol-3-yl)- 63% 2,5-Dihydroxy-3-(5-methoxy-1H-indol-3-yl)- [1,4]benzoquinone [1,4]benzoquinone Blue needles from benzene/hexane. mp 195–196° C. ¹H Dark green needles from acetone/hexane. mp 178° C. NMR(CDCl₃): 6.78(1H, d, J=2.4Hz), 6.93(1H, dd, J=2.1, (dec). ¹H NMR(d₆-acetone): 6.01(1H, s), 6.79(1H, 8.4Hz), 7.23(1H, s), 7.34(1H, d, J=9.0Hz), 7.54 dd, J=2.4, 8.7Hz), 7.06(1H, d, J=2.4Hz), 7.33 (1H, d, J=3.0Hz), 8.62(1H, br s). ¹³C NMR(d₆-acetone): (1H, d, J=8.7Hz), 7.56(1H, d, J=3.0Hz), 8.5–10.0 55.2, 103.8, 106.7, 112.4, 112.8, 126.5, 130.9, 131.0, 131.4, (2H, br s), 10.47(1H, br s). ¹³C NMR(d₆-acetone): 133.4, 139.8, 143.7, 154.8, 177.8, 178.0. IR(KBr): 3328, 55.1, 103.1, 104.0, 104.5, 111.8, 112.0, 127.4, 128.2, 3043, 2924, 2855, 1675, 1638, 1543, 1484, 1260, 1227cm⁻¹. 131.5, 154.1. IR(KBr): 3324, 3124, 3070, 3004, Anal. Calcd. for C₁₅H₉Cl₂NO₃: C, 55.93; H, 2.82; N, 4.35. 2959, 2924, 2838, 1629, 1481, 1354, 1208cm⁻¹. Found: C, 55.72; H, 2.99; N, 4.24. HRMS (EI): calcd. for C₁₅H₁₁NO₅ 285.0637, found 285.0639. 3-(5-Benzyloxy-1H-indol-3-yl)-2,5-dichloro- 63% 3-(5-Benzyloxy-1H-indol-3-yl)-2,5-dihydroxy- [1,4]benzoquinone [1,4]benzoquinone Blue needles from benzene/hexane. mp 164–165° C.. ¹H Dark green needles from acetone/hexane. mp 198° C. NMR(CDCl₃). 5.08(2H, s), 6.89(1H, s, J=2.1Hz), 7.01 (dec). ¹H NMR(d₆-acetone): 5.08(2H, s), 6.01(1H, 1H, dd, J=2.4, 8.7Hz), 7.22(1H, s), 7.26(1H, s), 7.30–7.50 s), 6.88(1H, dd, J=2.7, 8.7Hz), 7.20(1H, d, J=2.4Hz), (6H, m)7.53(1H, d, J=2.7Hz), 8.64(1H, br s). ¹³C NMR 7.37(1H, d, J=1.8Hz), 7.3–7.4(3H, m), 7.48 (d₆-acetone): 70.5, 105.6, 106.6, 112.8, 113.0, 126.3, 126.6, (2H, d, J=6.6Hz), 7.58(1H, d, J=2.7Hz), 8.0–10.2 127.8, 128.5, 131.0, 131.2, 131.7, 133.4, 138.1, 139.2, (2H, br s), 10.50(1H, br s). ¹³C NMR(d₆-acetone): 143.8, 153.9, 177.7, 178.0. IR(KBr): 3342, 3065, 3037, 70.5, 103.1, 104.6, 105.8, 112.0, 112.4, 127.4, 127.7, 2907, 2874, 1674, 1564, 1480, 1426, 1258, 1009cm⁻¹. Anal. 127.8, 128.4, 128.5, 131.7, 138.5, 158.3. IR(KBr): Calcd. for C₂₁H₁₃Cl₂NO₃: C, 63.34; H, 3.29; N, 3.52. Found: 3110, 3035, 2919, 2866, 1632, 1479, 1358, 1195, C, 63.24; H, 3.42; N, 3.49. 1018, 930cm⁻¹. HRMS (EI): calcd. for C₂₁H₁₅NO₅ 361.0950, found 361.0951. 2,5-Dichloro-3-(5-methyl-1H-indol-3-yl)-[1,4]benzoquinone 80% 2,5-Dihydroxy-3-(5-methyl-1H-indol-3-yl)- Blue needles from benzene/hexane. mp 176–177° C.. ¹H [1,4]benzoquinone NMR(d₆-acetone): 2.40(3H, s), 7.02(1H, dd, J=1.8, 8.4Hz), Dark green needles from acetone/hexane. mp 190° C. 7.20(1H, m), 7.33(1H, s), 7.40(1H, d, J=8.4Hz), (dec). ¹H NMR(d₆-acetone): 2.09(3H, s), 6.02(1H, 7.66(1H, dd, J=3.0Hz), 10.89(1H, br s). ¹³C NMR(d₆- s), 6.96(1H, dd, J=1.6, 8.4Hz), 7.31(1H, s), 7.32 acetone): 21.27, 106.4, 111.9, 121.4, 123.9, 126.2, 129.3, (1H, d, J=8.4Hz), 7.53(1H, d, J=2.8Hz), 8.2–10.2 130.6, 133.4, 134.8, 136.4, 139.3, 143.6, 177.8, 177.9. IR (2H, br s), 10.46(1H, br s). ¹³C NMR(d₆-acetone): (KBr): 3367, 3069, 2919, 2859, 1657, 1654, 1561, 1507, 21.1, 103.1, 104.2, 111.2, 112.2, 121.6, 123.1, 127.2, 1423, 1248, 1111cm⁻¹. Anal. Calcd. for C₁₅H₉Cl₂NO₂: C, 127.7, 128.0, 134.8. IR(KBr): 3305, 3045, 1631, 58.85; H, 2.96. Found: C, 58.95; H, 3.14. 1350, 1232, 1203, 932, 798cm⁻¹. HRMS (EI): calcd. for C₁₅H₁₁NO₄ 269.0688, found 269.0683. 3-(6-Fluoro-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone 80% 3-(6-Fluoro-1H-indol-3-yl)-2,5-dihydroxy- Blue needles from benzene/hexane. mp 168–169° C.. ¹H [1,4]benzoquinone NMR(d₆-acetone): 6.95(1H, tt, J=1.8, 9Hz), 7.28(1H, dt, Dark green needles from acetone/hexane. mp 130–131° C.. J=1.8, 9.6Hz), 7.37(1H, d, J=2.1Hz), 7.40(1H, d, J=5.4, ¹H NMR(d₆-acetone): 6.03(1H, s), 6.85 8.7Hz), 7.72(1H, d, J=2.4Hz), 11.12(1H, br s). ¹³C (1H, dt, J=2.4, 9.6Hz), 7.19(1H, dd, J=2.4, 9.9Hz), NMR(d₆-acetone): 98.1, 98.4, 106.8, 108.6, 108.9, 122.6, 7.51(1H, dd, J=5.4, 9.0Hz), 7.60(1H, d, J=2.7Hz), 122.7, 131.0, 133.4, 143.8, 158.2, 161.4, 177.4, 177.9. IR 8.2–10.4(2H, br s), 10.67(1H, br s). ¹³C (KBr): 3380, 3079, 2920, 1650, 1559, 1453, 1409, 1235, NMR(d₆-acetone): 97.2, 97.6, 103.2, 107.5, 107.8, 1140, 1010, 882cm⁻¹. Anal. Calcd. for C₁₄H₆Cl₂FNO₂: C, 122.9, 126.0, 123.6, 128.2, 136.2, 158.1, 161.2. IR 54.22; H, 1.95. Found: C, 54.48; H, 2.06. (KBr): 3350, 1626, 1528, 1454, 1361, 1235cm⁻¹. HRMS (EI): calcd. for C₁₄H₈FNO₄ 273.0437, found 273.0436. 3-(6-Chloro-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone 71% 3-(6-Chloro-1H-indol-3-yl)-2,5-dihydroxy- Blue needles from benzene/hexane. mp 182–183° C.. ¹H [1,4]benzoquinone NMR(CDCl₃): 7.18(1H, dd, J=1.8, 8.7Hz), 7.23(1H, s), Dark green needles from acetone/hexane. mp 229° C. 7.26(1H, s), 7.31(1H, d, J=8.7Hz), 7.44(1H, dd, J=0.6, (dec). ¹H NMR(d₆-acetone): 6.03(1H, s), 7.04(1H, 1.8Hz), 7.54(1H, d, J=2.7Hz), 8.75(1H, br s). ¹³C NMR dd, J=1.8, 8.7Hz), 7.50(1H, d, J=1.8Hz), 7.52 (d₆-acetone): 106.9, 112.0, 117.9, 120.7, 122.8, 124.7, 1H, d, J=8.7Hz), 7.64(1H, d, J=2.4Hz), 8.4–10.4 127.7, 131.3, 133.4, 136.8, 138.6, 143.8, 177.4, 177.8. IR (2H, br s), 10.74(1H, br s). ¹³C NMR(d₆-acetone). (KBr): 3325, 3122, 3071, 2962, 2926, 1673, 1564, 1517, 103.2, 105.0, 111.3, 111.4, 119.6, 123.2, 125.6, 1449, 1401, 1020cm⁻¹. Anal. Calcd. for C₁₄H₆Cl₃NO₂: C, 127.0, 128.6, 136.8. IR(KBr): 3418, 3305, 2956, 51.49; H, 1.85; N, 4.29. Found: C, 51.77; H, 1.94; N, 4.21. 1623, 1534, 1451, 1357, 936cm⁻¹. HRMS (EI): calcd. for C₁₄H₈ClNO₄ 289.0142, found 289.0144. 3-(6-Bromo-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone 83% 3-(6-Bromo-1H-indol-3-yl)-2,5-dihydroxy- Blue needles from benzene/hexane. mp 180° C.(dec). ¹H [1,4]benzoquinone NMR(d₆-acetone): 5.15(2H, s), 6.90(1H, dd, J=2.4, 9.0Hz), Dark green needles from acetone/hexane. mp 205° C. 7.14(1H, d, J=2.1Hz), 7.26–7.36(3H, m), 7.39(2H, (dec). ¹H NMR(d₆-acetone): 5.14(2H, s), 6.01(1H, t, J=7.2Hz), 7.50(2H, d, J=7.2Hz), 7.61(1H, d, J=2.4Hz), s), 6.80(1H, dd, J=2.4, 8.7Hz), 7.07(1H, d, J=2.1Hz), 10.84(1H, br s). ¹³C NMR(d₆-acetone): 70.2, 96.6, 7.26–7.58(9H, m), 8.4–10.2(2H, br s), 10.44 106.9, 111.1, 120.4, 122.4, 122.9, 127.6, 127.8, 128.6, (1H, br s). ¹³C NMR(d₆-acetone): 70.2, 96.0, 103.1, 129.6, 129.8, 133.4, 137.2, 138.0, 143.7, 155.8, 177.4, 104.7, 110.1, 121.5, 122.7, 126.7, 127.6, 127.7, 177.9. IR(KBr): 3396, 3127, 3064, 2946, 2815, 1672, 1651, 128.5, 137.0, 138.2, 155.4. IR(KBr): 3414, 3314, 1560, 1237, 1011, 819cm⁻¹. Anal. Calcd. for C₂₁H₁₃Cl₂NO₃: 2925, 1631, 1353, 1299, 1239, 1023, 935cm⁻¹. C, 63.34; H, 3.29; N, 3.52. Found: C, 63.07; H, 3.43; N, HRMS (EI): calcd. for C₂₁H₁₅NO₅ 361.0950, found 3.45. 361.0951. 2,5-Dichloro-3-(6-methyl-1H-indol-3-yl)-[1,4]benzoquinone 82% 2,5-Dihydroxy-3-(6-methyl-1H-indol-3-yl)- This compound was synthesized according to the general [1,4]benzoquinone procedure A from 7-methyl-1H-indole(100 mg, 0.762 mmol) Dark green needles from acetone/hexane. mp 190° C. and 2,5-dichloro-1,4-benzoquinone(270 mg, 1.52 mmol) (dec). ¹H NMR(d₆-acetone): 2.37(3H, s), 5.97(1H, and was obtained as a blue solid(188 mg, 81%). mp s), 6.84(1H, dd, J=1.6, 8.4Hz), 7.20(1H, s), 7.38 169–170° C.. ¹H NMR(d₆-acetone): 2.39(3H, s), 6.92(1H, (1H, d, J=8.4Hz), 7.48(1H, d, J=2.8Hz), 8.0–10.0 dd, J=0.8, 4.4Hz), 7.25(1H, d, J=8.4Hz), 7.24–7.26(2H, (2H, br s), 10.45(1H, br s). ¹³C NMR(d₆-acetone): m), 7.61(1H, d, J=2.8Hz), 10.92(1H, br s). ¹³C NMR(d₆- 21.0, 103.2, 104.6, 111.3, 112.3, 121.0, 121.7, 124.8, acetone): 20.9, 106.6, 112.0, 121.5, 122.1, 123.8, 130.3, 127.1, 130.9, 136.8. IR(KBr): 3398, 2919, 2855, 131.9, 133.4, 136.2, 136.9, 139.2, 143.7, 177.7, 178.0. IR 1701, 1617, 1530, 1452, 932cm⁻¹. HRMS (EI): (KBr): 3409, 3065, 2918, 1652, 1557, 1256, 1236, 1116, calcd. for C₁₅H₁₁NO₄ 269.0688, found 269.0685. 1015, 878, 805cm⁻¹. Anal. Calcd. for C₁₅H₉Cl₂NO₂: C, 58.85; H, 2.96. Found: 58.94; H, 3.08. 2,5-Dichloro-3-(7-propyl-1H-indol-3-yl)-[1,4]benzoquinone 66% 2,5-Dihydroxy-3-(7-propyl-1H-indol-3-yl)- Blue needles from benzene/hexane. mp 187–188° C.. R_(f) =0.31 [1,4]benzoquinone (3:7, EtOAc/hexane). ¹H NMR(CDCl₃)δ 8.70(bs, Green crystals from benzene/hexane. mp 218–219° C.. NH), 7.55(d, J=3.0Hz, 1H), 7.32(m, 1H), 7.21(s, 1H), IR(KBr): 3427, 3326, 1618, 1325, 1291, 1185cm⁻¹. 7.11(m, 2H), 2.53(s, 3H). ¹H NMR(d₆-acetone): δ 10.95 ¹H NMR(d₆-acetone): δ 10.58(1H, br s), 9.0–10.0 (1H, br s), 7.68(1H, m), 7.37(1H, m), 7.25(1H, m), 7.03 (2H, br), 7.56(1H, d, J=2.7Hz), 7.36(1H, t, J=4.5Hz), (2H, m), 2.55(3H, s). ¹³C NMR(d₆-acetone): δ 178.0, 6.95(1H, s), 6.93(1H, s), 6.02(1H, s), 177.7, 143.7, 139.3, 136.3, 135.9, 133.4, 130.0, 125.7, 2.52(3H, s). ¹³C NMR(d₆-acetone): δ 135.8, 127.2, 122.9, 121.5, 120.5, 119.4, 107.2, 16.2. IR(KBr): 3403, 126.6, 122.1, 120.6, 119.6, 119.5, 112.2, 105.2, 3072, 1670, 1563, 1431, 1236, 1037, 750cm⁻¹. Anal. Calcd. 103.2, 16.4. HRMS (FAB) Calcd. For C₁₅H₁₁NO₄ for C₁₅H₉Cl₂NO₂: C, 58.85; H, 2.97; N, 4.58. Found: C, [M⁺]: 269.0688. Found: 269.0686. 58.62; H, 2.78; N, 4.53. 2,5-Dichloro-3-(7-methyl-1H-indol-3-yl)-[1,4]benzoquinone 73% 2,5-Dihydroxy-3-(7-methyl-1H-indol-3-yl)- Blue needles from benzene/hexane. mp 140–141° C.. ¹H [1,4]benzoquinone NMR(d₆-acetone): 1.00(3H, t, J=7.2Hz), 1.77(2H, h, J=7.5Hz), Dark green needles from acetone/hexane. mp 207° C. 2.93(2H, dd, J=9.0, 16.5Hz), 7.03(1H, s), 7.06 (dec). ¹H NMR(d₆-acetone): 0.98(3H, t, J=7.6Hz), (1H, t, J=7.2Hz), 7.24(1H, dd, J=1.5, 6.9Hz), 7.34(1H, 1.76(2H, h, J=7.6Hz), 2.89(1H, t, J=7.6Hz), s), 7.67(1H, dd, J=1.2, 3.0Hz), 11.09(1H, br s). ¹³C NMR 6.02(1H, s), 6.94–7.00(2H, m), 7.39–7.39(1H, m), (d₆-acetone): 13.9, 23.6, 33.4, 107.2, 119.5, 120.5, 122.1, 7.54(1H, d, J=2.8Hz), 8.4–10.0(2H, br s), 10.58 125.9, 126.3, 130.1, 133.4, 135.4, 136.5, 139.2, 143.7, 1H, br s). ¹³C NMR(d₆-acetone): 13.7, 23.3, 33.3, 177.6, 177.9. IR(KBr): 3401, 3062, 2958, 2929, 2871, 103.2, 105.1, 112.2, 119.4, 119.7, 121.4, 125.4, 2858, 1670, 1572, 1434, 1115, 1023cm⁻¹. Anal. Calcd. for 126.9, 127.2, 134.3. IR(KBr): 3421, 3330, 2955, C₁₇H₁₃Cl₂NO₂: C, 61.10; H, 3.92. Found: C, 60.94; H, 4.13. 2868, 1615, 1534, 1354, 1297, 1230, 1195cm⁻¹. HRMS (EI): calcd. for C₁₇H₁₅NO₄ 297.1001, found 297.1001. 3-(7-tert-butyl-1H-indol-3-yl)-2,5-dichloro- 83% 3-(7-tert-butyl-1H-indol-3-yl)-2,5-dihydroxy- [1,4]benzoquinone [1,4]benzoquinone Blue needles from benzene/hexane. mp 189–190° C.. ¹H Dark green needles from acetone/hexane. mp 210–211° C.. NMR(d₆-acetone): 1.54(9H, s), 7.06(1H, t, J=7.8Hz), ¹H NMR(d₆-acetone): 1.53(9H, s), 6.03 7.15(1H, d, J=6.6Hz), 7.27(1H, d, J=7.8Hz), 7.37(1H, (1H, s), 6.98(1H, t, J=8.0Hz), 7.08(1H, dd, J=1.2, s), 7.64(1H, d, J=3.3Hz), 10.77(1H, br s). ¹³C NMR(d₆- 7.6Hz), 7.36(1H, d, J=8.0Hz), 7.51(1H, d, J=2.8Hz), acetone): 34.5, 106.8, 118.9, 119.3, 120.3, 127.0, 129.5, 9.0–10.0(2H, br s), 10.35(1H, br s). ¹³C 133.4, 133.6, 134.4, 137.0, 139.3, 143.7, 177.6, 177.9. IR NMR(d₆-acetone): 29.8, 34.2, 103.2, 104.6, 112.0, (KBr): 3438, 3064, 2963, 1659, 1565, 1422, 1269, 1113, 118.1, 119.3, 120.1, 126.8, 128.0, 133.5, 133.6. IR 1026, 750cm⁻¹. Anal. Calcd. for C₁₈H₁₅Cl₂NO₂: C, 62.08; (KBr): 3337, 2967, 2866, 1631, 1360, 1299, 1223, H, 4.34. Found: C, 62.32; H, 4.58. 931cm⁻¹. HRMS (EI): calcd. for C₁₈H₁₇NO₄ 311.1158, found 311.1158. 2,5-Dichloro-3-(7-phenyl-1H-indol-3-yl)-[1,4]benzoquinone 76% 2,5-Dihydroxy-3-(7-phenyl-1H-indol-3-yl)- Blue needles from benzene/hexane. mp 185–186° C.. ¹H [1,4]benzoquinone NMR(d₆-acetone): 7.21–7.25(2H, m), 7.35(1H, s), 7.38–7.44 Dark green needles from acetone/hexane. mp 210–211° C.. (2H, m), 7.47–7.53(2H, m), 7.66(2H, dd, J=1.2, 8.4Hz), ¹H NMR(d₆-acetone): 6.04(1H, s), 7.1–7.2 7.70(1H, d, J=2.8Hz), 10.94(1H, br s). ¹³C NMR (2H, m), 7.40(1H, t, J=7.4Hz), 7.4–7.7(7H, m), (d₆-acetone): 118.0, 119.0, 120.5, 120.9, 121.1, 122.5, 9.0–10.2(2H, br s), 10.58(1H, br s). ¹³C NMR(d₆- 126.7, 127.7, 128.7, 129.2, 130.9, 132.6, 133.5, 133.9, 138.9, acetone): 103.3, 105.1, 119.9, 121.3, 121.4, 121.7, 139.1, 143.8, 143.8, 177.6, 178.0. IR(KBr): 3424, 3328, 125.9, 127.4, 127.9, 128.1, 128.6, 129.1, 133.8, 3137, 3069, 3029, 1675, 1651, 1568, 1426, 755cm⁻¹. Anal. 139.4. IR(KBr): 3402, 3325, 3029, 2950, 2923, Calcd. for C₂₀H₁₁Cl₂NO₂: C, 65.24; H, 3.01; N, 3.80. Found: 1633, 1524, 1428, 1343, 760cm⁻¹. HRMS (EI): C, 65.44; H, 3.44; N, 3.60. calcd. for C₂₀H₁₃NO₄ 331.0845, found 331.0844. 2,5-Dichloro-3-(7-methoxy-1H-indol-3-yl)- 75% 2,5-Dihydroxy-3-(7-methoxy-1H-indol-3-yl)- [1,4]benzoquinone [1,4]benzoquinone Blue needles from benzene/hexane. mp 183–184° C.. ¹H Dark green needles from acetone/hexane. mp 202–203° C.. NMR(CDCl₃): 3.94(3H, s), 6.67(1H, d, J=7.6Hz), 6.96 ¹H NMR(d₆-acetone): 3.91(3H, s), 5.98 (1H, dd, J=0.8, 8.0Hz), 7.09(1H, t, J=8.0Hz), 7.17(1H, (1H, s), 6.64(1H, d, J=5.7Hz), 6.91(1H, t, J=6.0Hz), s), 7.50(1H, d, J=3.2Hz), 8.94(1H, br s). ¹³C NMR(d₆- 7.09(1H, d, J=6.0Hz), 7.51(1H, d, J=1.8Hz), acetone): 55.1, 102.5, 107.2, 114.4, 120.9, 126.7, 127.4, 7.8–10.2(2H, br s), 10.63(1H, br s). ¹³C NMR 129.8, 133.4, 136.7, 139.2, 143.8, 146.9, 177.6, 178.0. IR (d₆-acetone): 55.0, 101.7, 103.2, 105.2, 112.1, 114.8, (KBr): 3391, 3152, 1667, 1626, 1499, 1419, 1114, 1013. 119.7, 126.7, 127.1, 128.4, 146.6. IR(KBr): 3390, Anal. Calcd. for C₁₅H₉Cl₂NO₃: C, 55.93; H, 2.82; N, 4.35. 3311, 2928, 1634, 1501, 1449, 1354, 1097, 933cm^(−1.) Found: C, 56.16; H, 2.94; N, 4.23. HRMS (EI): calcd. for C₁₅H₁₁NO₅ 285.0637, found 285.0636. 3-(7-Benzyloxy-1H-indol-3-yl)-2,5-dichloro- 84% 3-(7-Benzyloxy-1H-indol-3-yl)-2,5-dihydroxy- [1,4]benzoquinone [1,4]benzoquinone Blue needles from benzene/hexane. mp 174–175° C.. ¹H Dark green needles from acetone/hexane. mp 202° C. NMR(d₆-acetone): 5.29(2H, s), 6.85(1H, dd, J=1.6, 6.8Hz), (dec). ¹H NMR(d₆-DMSO): 5.23(2H, s), 5.84(1H, 6.99–7.07(2H, m), 7.35(1H, t, J=7.2Hz), 7.36(1H, s), 6.38(1H, d, J=7.6Hz), 6.81(1H, d, J=8.0Hz), s), 7.42(2H, dt, J=1.2, 7.2Hz), 7.57(1H, d, J=7.2Hz), 6.87(1H, d, J=8.0Hz), 7.26–7.32(2H, m), 7.36 7.66(1H, d, J=2.8Hz), 11.25(1H, br s). ¹³C NMR(d₆- (2H, t, J=7.2Hz), 7.52(2H, d, J=7.6Hz), 9.0–10.0 acetone): 70.0, 103.8, 107.2, 114.6, 120.8, 127.0, 127.6, (2H, br s), 11.42(1H, br s). ¹³C NMR(d₆-DMSO): 127.97, 128.0, 128.6, 129.7, 129.8, 133.4, 137.6, 139.2, 69.8, 103.5, 104.4, 105.5, 112.7, 115.0, 119.6, 126.7, 143.8, 146.0, 177.6, 178.0. IR(KBr): 3380, 3066, 2918, 127.5, 128.2, 128.4, 128.8, 129.1, 138.0, 145.6. IR 2872, 1674, 1657, 1567, 1428, 1244, 1111cm⁻¹. Anal. KBr): 3421, 2960, 2925, 2856, 1631, 1501cm⁻¹. Calcd. for C₂₁H₁₃Cl₂NO₃: C, 63.34; H, 3.29. Found: C, HRMS (EI): calcd. for C₂₁H₁₅NO₅ 361.0950, found 63.50; H, 3.38. 361.0950. 3-(7-Fluoro-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone 75% 3-(7-Fluoro-1H-indol-3-yl)-2,5-dihydroxy- Blue needles from benzene/hexane. mp 177–178° C.. ¹H [1,4]benzoquinone NMR(d₆-acetone): 6.98(1H, dd, J=8.0, 11.2Hz), 7.09 Dark blue needles from acetone/hexane. mp 201° C. (1H, dt, J=4.8, 8.0Hz), 7.24(1H, d, J=8.0Hz), 7.40(1H, (dec). IR(KBr): 3420, 3277, 3162, 2923, 2861, 1632, s), 7.76(1H, d, J=2.8Hz), 11.41(1H, br s). ¹³C NMR(d₆- 1357, 1299, 1234cm⁻¹. ¹H NMR(d₆-acetone): 6.03 acetone): 106.8, 107.0, 117.6, 120.5, 120.6, 130.6, 130.8, (1H, s), 6.90(1H, dd, J=8.0, 11.6Hz), 6.99(1H, dt, 131.1, 132.6, 133.4, 138.8, 143.9, 177.5, 177.9. IR(KBr): J=4.8, 8.0Hz), 7.34(1H, d, J=8.0Hz), 7.65(1H, J=2.8Hz), 3395, 3096, 1671, 1573, 1503, 1423, 1265, 1236, 1109, 9.0–10.2(2H, br s), 11.00(1H, br s). ¹³C 1038cm⁻¹. Anal. Calcd. for C₁₄H₆Cl₂FNO₂: C, 54.22; H, NMR(d₆-acetone): 103.3, 106.0, 106.2, 111.3, 118.0, 1.95. Found: C, 54.36; H, 2.09. 119.3, 119.4, 128.1, 128.3, 130.7. HRMS (EI): calcd. for C₁₄H₈FNO₄ 273.0437, found 273.0440. 3-(7-Chloro-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone 70% 3-(7-Chloro-1H-indol-3-yl)-2,5-dihydroxy- Blue needles from benzene/hexane. mp 213–214° C.. ¹H [1,4]benzoquinone Dark blue needles from NMR(d₆-acetone): 7.14(1H, t, J=8.0Hz), 7.27(1H, dd, J=0.8, acetone/hexane. mp 207° C.(dec). ¹H NMR(d₆- 7.6Hz), 7.40(1H, s), 7.41(1H, dd, J=0.8, 8.0Hz), acetone): 6.04(1H, s), 7.04(1H, t, J=10.4Hz), 7.20 7.77(1H, d, J=3.2Hz), 11.23(1H, br s). ¹³C NMR(d₆- (1H, d, J=10.0Hz), 7.66(1H, d, J=4.0Hz), 9.0–10.2 acetone): 108.2, 120.8, 121.4, 122.0, 128.0, 130.9, 131.0, (2H, br s), 10.86(1H, br s). ¹³C NMR(d₆- 133.7, 138.9, 144.1, 177.6, 178.1. IR(KBr): 3406, 3071, acetone): 103.4, 106.1, 111.2, 116.4, 120.1, 120.9, 2950, 1678, 1649, 1542, 1508, 1434, 1247, 1204cm⁻¹. Anal. 121.0, 128.4, 128.7, 133.3. IR(KBr): 3428, 3310, Calcd. for C₁₄H₆Cl₃NO₂: C, 51.49; H, 1.85; N, 4.29. Found: 3091, 1626, 1354, 1296, 1063, 930cm⁻¹. HRMS C, 51.59; H, 2.01; N, 4.28. (EI): calcd. for C₁₄H₈ClNO₄ 289.0142, found 289.0138. 3-(7-Bromo-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone 71% 3-(7-Bromo-1H-indol-3-yl)-2,5-dihydroxy- Blue needles from benzene/hexane. mp 191–192° C.. ¹H [1,4]benzoquinone NMR(d₆-acetone): 7.09(1H, t, J=8.0Hz), 7.41(1H, s), Dark blue needles from acetone/hexane. mp 225–226° C.. 7.44(2H, dt, J=0.8, 8.0Hz), 7.77(1H, d, J=1.6Hz), ¹H NMR(d₆-acetone): 6.04(1H, s), 6.99(1H, t, J=7.8Hz), 11.14(1H, br s). ¹³C NMR(d₆-acetone): 104.8, 108.0, 7.35(1H, dd, J=0.6, 7.8Hz), 7.54(1H, d, 121.0, 121.6, 124.9, 127.5, 130.6, 130.7, 133.4, 138.1, J=8.1Hz), 7.66(1H, d, J=2.7Hz), 8.4–10.0(2H, 138.7, 143.9, 177.4, 178.0. IR(KBr): 3368, 3160, 3060, br s), 10.76(1H, br s). ¹³C NMR(d₆-acetone): 103.4, 2972, 2866, 1662, 1561, 1262, 1098, 1012, 882cm⁻¹. Anal. 104.3, 106.2, 111.3, 120.6, 121.4, 124.1, 128.4, Calcd. for C₁₄H₆BrCl₂NO₂: C, 45.32; H, 1.63; N, 3.78. 130.8, 134.7. IR(KBr): 3330, 2959, 2929, 2854, Found: C, 45.47; H, 1.75; N, 3.81. 1617, 1531, 1433, 1354, 1292, 932cm⁻¹. HRMS (EI): calcd. for C₁₄H₈BrNO₄ 332.9637, found 332.9637. 3-(7-Benzyl-1H-indol-3-yl)-2,5-dichloro-[1,4]benzoquinone 85% 3-(7-Benzyl-1H-indol-3-yl)-2,5-dichloro- Blue needles from benzene/hexane. mp 159–160° C.. ¹H [1,4]benzoquinone NMR(d₆-acetone): 4.30(2H, s), 7.01(1H, d, J=6.6Hz), Dark green needles from acetone/hexane. mp 206–207° C.. 7.08(1H, t, J=7.2Hz), 7.14–7.22(1H, m), 7.24–7.33(4H, ¹H NMR(d₆-acetone): 4.28(2H, s), 6.02 m), 7.35(1H, s), 7.66(1H, d, J=3.0Hz), 10.95(1H, s). ¹³C (1H, s), 6.92–7.03(2H, m), 7.14–7.34(5H, m), 7.41 NMR(d₆-acetone): 37.0, 107.3, 117.8, 120.0, 120.6, 122.5, (1H, d, J=7.5Hz), 7.54(1H, d, J=2.7Hz), 8.6–10.3 122.9, 125.0, 126.3, 128.6, 129.0, 130.2, 132.5, 133.4, (2H, br s), 10.49(1H, br s). ¹³C NMR(300Hz, d₆- 135.3, 140.3, 143.7, 177.5, 177.9. IR(KBr): 3414, 3059, acetone): 37.1, 103.2, 105.2, 112.0, 119.6, 120.2, 1676, 1656, 1566, 1434, 1266, 1237, 1113, 1033cm⁻¹. Anal. 122.2, 124.1, 126.1, 127.1, 127.4, 128.5, 128.9, Calcd. for C₂₁H₁₃Cl₂NO₂: C, 65.99; H, 3.43; N, 3.66. Found: 135.2, 140.7. IR(KBr): 3422, 3326, 3084, 3027, C, 65.74; H, 3.49; N, 3.71. 1612, 1532, 1438, 1353cm⁻¹. HRMS (EI): calcd. for C₂₁H₁₅NO₄ 345.1001, found 345.1001. 2,5-Dichloro-3-[7-(2-methyl-benzyl)-1H-indol-3-yl]- 96% 2,5-Dihydroxy-3-[7-(2-methyl-benzyl)-1H-indol-3- [1,4]benzoquinone yl]-[1,4]benzoquinone Blue needles from benzene/hexane. mp 101–102° C.. ¹H Dark green needles from acetone/hexane. mp 220° C. NMR(d₆-acetone): 2.27(3H, s), 4.28(2H, s), 6.75(1H, d, J=6.9Hz), (dec). ¹H NMR(d₆-acetone): 2.27(3H, s), 4.25(2H, 7.00–7.23(5, m), 7.30(1H, d, J=8.1Hz), 7.33) s), 6.02(1H, s), 6.70(1H, d, J=7.2Hz), 6.92–7.04 (1H, s), 7.68(1H, d, J=3.3Hz), 11.00(1H, br s). ¹³C NMR (2H, m), 7.05–7.15(2H, m), 7.18(1H, t, J=7.2Hz), (d₆-acetone): 19.1, 34.5, 107.3, 119.9, 120.1, 120.6, 122.0, 7.56(1H, d, J=2.4Hz), 8.8–10.2(2H, br s), 10.56 122.4, 124.1, 126.0, 126.2, 126.4, 129.4, 130.3, 133.4, (1H, br s). ¹³C NMR(d₆-acetone): 19.1, 34.6, 103.2, 135.5, 136.8, 138.0, 139.2, 143.7, 177.6, 177.9. IR(KBr): 105.2, 112.1, 119.5, 120.2, 121.6, 123.2, 126.1, 3414, 3060, 2920, 2857, 1656, 1561, 1433, 1237, 1110, 126.5, 126.9, 127.4, 129.4, 130.2, 135.4, 136.8, 1031cm⁻¹. Anal. Calcd. for C₂₂H₁₅Cl₂NO₁₂: C, 66.68; H, 138.3. IR(KBr): 3304, 2966, 1699, 1624, 1434, 3.82; N, 3.53. Found: C, 65.88; H, 3.85; N, 3.79. 1350, 1218cm⁻¹. HRMS (EI): calcd. for C₂₂H₁₇NO₄ 359.1158, found 359.1157. 3-(1H-Benzo[g]indol-3-yl)-2,5-dichloro-[1,4]benzoquinone 70% 3-(1H-Benzo[g]indol-3-yl)-2,5-dihydroxy- Blue needles from benzene/hexane. mp 210–211° C.. ¹H [1,4]benzoquinone NMR(d₆-acetone): 7.18–7.25(1H, m), 7.38–7.62(6H, m), Dark green needles from acetone/hexane. mp 210° C. 7.72–7.75(1H, m), 7.97(1H, d, J=8.1Hz), 8.49(1H, d, J=8.1Hz), (dec). ¹H NMR(d₆-acetone): 6.05(1H, s), 7.42(1H, 11.85(1H, br s). ¹³C NMR(d₆-acetone): 111.0, dt, J=1.5, 6.9Hz), 7.45–7.56(2H, m), 7.60–7.68 118.0, 119.8, 120.4, 120.9, 121.3, 124.0, 124.4, 125.9, (2H, m), 7.93(1H, d, J=8.1Hz), 8.36(1H, d, J=8.1Hz), 127.8, 128.7, 133.4. IR(KBr): 3340, 3063, 2922, 1675, 8.8–10.5(2H, br s), 11.51(1H, br s). ¹³C 1651, 1558, 1272, 1224, 1021, 871cm⁻¹. HRMS (FAB) : NMR(d₆-acetone): 103.2, 106.4, 111.9, 119.8, 120.3, calculated for C₁₈H₉Cl₂NO₂ 341.0010. Found 341.0013. 122.1, 122.4, 123.9, 125.4, 128.6, 130.5, 130.8. IR (KBr): 3318, 3158, 3060, 2923, 2855, 1612, 1353, 1291, 929, 814cm⁻¹. HRMS (FAB) : calcd. for C₁₈H₁₁NO₄ 305.0688, found 305.0686. 3-(2,6-Dimethyl-1H-indol-3-yl)-2,5-dichloro- 73% 3-(2,6-Dimethyl-1H-indol-3-yl)-2,5-dihydroxy- [1,4]benzoquinone [1,4]benzoquinone Blue needles from benzene/hexane. mp 114–115° C.. ¹H Dark green needles from acetone/hexane. mp 210° C. NMR(d₆-acetone): 2.34(3H, s), 2.39(3H, s), 6.86(1H, d, J=8.7Hz), (dec). ¹H NMR(d₆-acetone): 2.28(3H, s), 2.37(3H, 7.12(1H, d, J=8.1Hz), 7.17(1H, s), 7.37(1H, s), 6.03(1H, s), 6.78(1H, d, J=8.7Hz), 7.07–7.14 s), 10.46(1H, br s). ¹³C NMR(d₆-acetone): 13.0, 21.1, (2H, m), 8.4–9.9(2H, br s), 10.09(1H, br s). ¹³C 102.6, 111.0, 119.6, 121.5, 125.4, 130.9, 133.4, 136.4, NMR(d₆-acetone): 12.8, 21.2, 103.4, 110.5, 119.6, 136.8, 140.1, 144.0, 176.9, 178.1. IR(KBr): 3266, 3064, 120.7, 126.5, 129.9, 134.8, 136.4. IR(KBr): 3384, 2958, 2921, 1677, 1654, 1568, 1466, 1258, 1033cm⁻¹. Anal. 3324, 2975, 2912, 2857, 1637, 1352, 1295, 1228, Calcd. for C₁₆H₁₁Cl₂NO₂: C, 60.02; H, 3.46; N, 4.37. Found: 1184cm⁻¹. HRMS (EI): calcd. for C₁₆H₁₃NO₄ C, 59.76; H, 3.54; N, 4.32. 283.0845, found 283.0849. 3-(2,7-Dimethyl-1H-indol-3-yl)-2,5-dichloro- 84% 3-(2,7-Dimethyl-1H-indol-3-yl)-2,5-dihydroxy- [1,4]benzoquinone [1,4]benzoquinone Blue needles from benzene/hexane. mp 95–96° C.. ¹H NMR Dark green needles from acetone/hexane. mp 206° C. (d₆-acetone): 2.35(3H, s), 2.49(3H, s), 6.86–6.96(2H, m), (dec). ¹H NMR(d₆-acetone): 2.32(3H, s), 2.47(3H, 7.07(1H, d, J=6.9Hz), 7.36(1H, s), 10.51(1H, br s). ¹³C s), 6.05(1H, s), 6.85(1H, s), 6.88(1H, d, J=7.2Hz), NMR(d₆-acetone): 13.1, 16.4, 105.4, 117.6, 120.1, 120.4, 7.07(1H, dd, J=2.1, 7.2Hz), 8.5–10.0(2H, br 122.2, 127.2, 133.4, 135.4, 137.2, 139.8, 140.1, 144.0, s), 10.16(1H, br s). ¹³C NMR(d₆-acetone): 12.8, 176.9, 178.1. IR(KBr): 3362, 3064, 2919, 2860, 1675, 16.4, 102.6, 103.4, 112.0, 117.6, 119.2, 119.7, 121.3, 1620, 1453, 1269, 1243, 884cm⁻¹. Anal. Calcd. for 128.2, 135.2, 135.4. IR(KBr): 3331, 2945, 2856, C₁₆H₁₁Cl₂NO₂: C, 60.02; H, 3.46; N, 4.37. Found: C, 60.19; 1629, 1559, 1454, 1185, 932cm⁻¹. HRMS (EI): H, 3.59; N, 4.31. calcd. for C₁₆H₁₃NO₄ 283.0845, found 283.0846. 3-(6,7-Dimethyl-1H-indol-3-yl)-2,5-dichloro- 75% 3-(6,7-Dimethyl-1H-indol-3-yl)-2,5-dihydroxy- [1,4]benzoquinone [1,4]benzoquinone Blue needles from benzene/hexane. mp 184–185° C.. IR Dark green needles from acetone/hexane. mp 220° C. (KBr): 3387, 3071, 2920, 2860, 1675, 1551, 1506, 1433, (dec). ¹H NMR(d₆-acetone): 2.35(3H, s), 2.43(3H, 1263cm⁻¹. ¹H NMR(d₆-acetone)2.36(3H, s), 2.46(3H, s), s), 6.01(1H, s), 6.86(1H, d, J=8.1Hz), 7.25(1H, d, 6.95(1H, d, J=8.4Hz), 7.12(1H, d, J=8.0Hz), 7.33(1H, J=7.8Hz), 7.50(1H, d, J=2.7Hz), 8.8–10.2(2H, s), 7.61(1H, d, J=2.8Hz), 10.84(1H, s). ¹³C NMR(d₆- br s), 10.38(1H, br s). ¹³C NMR(d₆-acetone): 12.7, acetone): 12.6, 18.6, 107.2, 118.9, 119.2, 123.1, 124.0, 18.8, 103.1, 118.2, 112.5, 119.1, 122.2, 123.1, 125.0, 129.6, 130.0, 133.4, 136.6, 139.1, 143.6, 177.7, 178.0. Anal. 126.9, 128.6, 129.9. IR(KBr): 3340, 3150, 2921, Calcd. for C₁₆H₁₁Cl₂NO₂. C, 60.02; H, 3.46; N, 4.37. Found: 2869, 1615, 1533, 1351cm⁻¹. HRMS (EI): calcd. for C, 59.98; H, 3.59; N, 4.23 C₁₆H₁₃NO₄ 283.0845, found 283.0850. 3-(5,6-Methylenedioxy-1H-indol-3-yl)-2,5-dichloro- 57% 3-(5,6-Methylenedioxy-1H-indol-3-yl)-2,5- [1,4]benzoquinone dihydroxy-[1,4]benzoquinone Blue needles from benzene/hexane. mp 170° C.(dec). ¹H Dark green needles from acetone/hexane. mp 335° C. NMR(d₆-acetone): 5.95(2H, s), 6.82(1H, s), 6.99(1H, s), (dec). ¹H NMR(d₆-acetone): 5.91(2H, s), 6.00(1H, 7.34(1H, s), 7.54(1H, d, J=3.0Hz), 10.82(1H, br s). ¹³C s), 6.92(1H, d, J=0.8Hz), 6.94(1H, d, J=0.4Hz), NMR(d₆-acetone): 92.6, 100.3, 101.0, 107.1, 120.2, 128.9, 7.44(1H, d, J=2.8Hz), 8.8–10.2(2H, br s), 10.42 131.3, 133.3, 136.4, 139.2, 143.6, 143.7, 145.3, 177.6, (1H, br s). ¹³C NMR(d₆-acetone): 92.4, 100.6, 178.0. IR(KBr): 3337, 3066, 2971, 2874, 1668, 1566, 1426, 100.64, 100.8, 103.0, 109.8, 126.2, 126.3, 131.2, 1042, 942cm⁻¹. Anal. Calcd. for C₁₅H₇Cl₂NO₄: C, 53.60; H, 142.8, 144.7. IR(KBr): 3420, 3077, 2937, 2844, 2.10. Found: C, 53.61; H, 2.23. 1624, 1470, 1330cm⁻¹. HRMS (EI): calcd. for C₁₅H₉NO₆ 299.0430, found 299.0428. 3-(5,6-Dimethoxy-1H-indol-3-yl)-2,5-dichloro- 65% 3-(5,6-Dimethoxy-1H-indol-3-yl)-2,5-dihydroxy- [1,4]benzoquinone [1,4]benzoquinone Blue needles from benzene/hexane mp 186–187° C.. IR Dark green needles from acetone/hexane. mp 335° C. (KBr): 3300, 3040, 2952, 2957, 2829, 1634, 1546, 1484, (dec). ¹H NMR(d₆-acetone): 5.91(2H, s), 6.00(1H, 1228, 1087cm⁻¹. ¹H NMR(d₆-acetone): 3.78(3H, s), 3.84 s), 6.92(1H, d, J=0.8Hz), 6.94(1H, d, J=0.4Hz), (3H, s), 6.93(1H, s), 7.10(1H, s), 7.36(1H, s), 7.56(1H, d, 7.44(1H, d, J=2.8Hz), 8.8–10.2(2H, br s), 10.42 J=2.7Hz), 10.82(1H, br s). ¹³C NMR(d₆-acetone): 55.7, (1H, br s). ¹³C NMR(d₆-acetone): 92.4, 100.6, 56.0, 95.8, 104.7, 106.8, 117.6, 119.0, 120.6, 129.1, 130.8, 100.64, 100.8, 103.0, 109.8, 126.2, 126.3, 131.2, 133.4, 143.6, 145.9, 147.9, 177.7, 177.9. Anal. Calcd. for 142.8, 144.7. IR(KBr): 3420, 3077, 2937, 2844, C₁₆H₁₁Cl₂NO₄: C, 54.57; H, 3.15. Found: C, 51.03; H, 3.32. 1624, 1470, 1330cm⁻¹. HRMS (EI): calcd. for C₁₅H₉NO₆ 299.0430, found 299.0428.

EXAMPLE 7 Inhibition of Phosphatase Cdc25B

Using procedures known to those skilled in the art, the compounds listed in Table 3 were screened against Cdc25A, Cdc25B, and Cdc25C at 50 μM. Similar amounts of inhibition were seen for all three isoforms of Cdc25. To analyze these effects further, the compounds were then screened against Cdc25B in a 3 pt. IC-50, using 1, 7 and 50 μM. Several of the compounds were also screened with an 8 pt. IC-50 process, using 0.1–10 μM, allowing determination of Hill slopes. Further data (not shown) demonstrates that these compounds are competitive vs. substrate, indicating that they bind at the active site of Cdc25. Overall, these results show that these are preferred compound for treating a Cdc25-related cell proliferative disorder.

TABLE 3 Percent Inhibition at plate IC50 IC50 50 uM compound CHEMISTRY No. Ser. No. (3 pt) (8 pt) Cdc25 A Cdc25 B Cdc25 C 2,5-dihydroxy-3(1H-indol-3-yl)-[1,4]- 2A ZL-I-197 14.2 16.5 55 75 69 benzoquinone 2,5-dihydroxy-3-(1-methyl-1H-indol-3-yl)-[1,4]- 2B ZL-I-194 184.5 63 42 19 benzoquinone 2,5-dihydroxy-3-(2-methyl-1H-indol-3-yl)-[1,4]- 2C ZL-I-186 138.3 35 9 5 benzoquinone 2,5-dihydroxy-3-(2-ethyl-1H-indol-3-yl)-[1,4]- 2D ZL-I-174 33.4 51 36 14 benzoquinone 2,5-dihydroxy-3-(2-cyclopropyl-1H-indol-3-yl)- 2E ZL-I-184 2.3 24.4 79 32 0 [1,4]-benzoquinone 2,5-dihydroxy-3-(2-isopropyl-1H-indol-3-yl)-[1,4]- 2F ZL-I-185 8.3 58 13 0 benzoquinone 2,5-dihydroxy-3-[2-(1-methylcycloproyl)-1H- 2G LD-I-205 3.6 49 17 0 indol-3-yl]-[1,4]-benzoquinone 2,5-dihydroxy-3-(2-tert-butyl-1H-indol-3-yl)-[1,4]- 2H ZL-I-187 13.9 31 11 0 benzoquinone 2,5-dihydroxy-3-[2-(1-methylcyclohexyl)-1H- 3A LD24B 82.9 69 11 22 indol-3-yl]-[1,4]-benzoquinone 2,5-dihydroxy-3-(2-phenyl-1H-indol-3-yl)-[1,4]- 3B ZL-I-207 22.2 93 38 18 benzoquinone 2,5-dihydroxy-3-[2-(1,1-dimethyl-allyl)-1H-indol- 3C ZL-I-202 40.3 40 0 21 3-yl]-[1,4]-benzoquinone 2,5-dihydroxy-3-(4-fluoro-1H-indol-3-yl)-[1,4]- 3D LD-I-217 1.2 15.9 59 5 0 benzoquinone 2,5-dihydroxy-3-(4-chloro-1H-indol-3-yl)-[1,4]- 3E LD9B 3.0 8.4 67 21 0 benzoquinone 2,5-dihydroxy-3-(4-bromo-1H-indol-3-yl)-[1,4]- 3F LD11B 3.1 >2 0 6 54 benzoquinone 2,5-dihydroxy-3-(4-methoxy-1H-indol-3-yl)-[1,4]- 3G LD19B 7.2 77 21 19 benzoquinone 2,5-dihydroxy-3-(4-benzyloxy-1H-indol-3-yl)- 3H LD-I-90 4.6 87 0 2 [1,4]-benzoquinone 2,5-dihydroxy-3-(4-methyl-1H-indol-3-yl)-[1,4]- 4A LD13B 6.0 51 9 28 benzoquinone 2,5-dihydroxy-3-(5-fluoro-1H-indol-3-yl)-[1,4]- 4B LD-I-204 11.7 64 32 23 benzoquinone 2,5-dihydroxy-3-(5-chloro-1H-indol-3-yl)-[1,4]- 4C LD3B 2.3 95 32 45 benzoquinone 2,5-dihydroxy-3-(5-bromo-1H-indol-3-yl)-[1,4]- 4D LD6B 2.4 96 55 26 benzoquinone 2,5-dihydoxy-3-(7-methyl-1H-indol-3-yl)- 4E ZL-III-198 69.3 10 0 0 [1,4]naphthoquinone 2,5-dihydroxy-3-(5-methoxy-1H-indol-3-yl)-[1,4]- 4F LD2B 6.0 15 0 0 benzoquinone 2,5-dihydroxy-3-(5-benzyloxy-1H-indol-3-yl)- 4G LD4B 1.8 96 50 26 [1,4]-benzoquinone 2,5-dihydroxy-3-(5-methyl-1H-indol-3-yl)-[1,4]- 4H LD20B 2.5 94 61 55 benzoquinone 2,5-dihydroxy-3-(6-fluoro-1H-indol-3-yl)-[1,4]- 5A LD-I-210 2.0 6.8 91 48 90 benzoquinone 2,5-dihydroxy-3-(6-chloro-1H-indol-3-yl)-[1,4]- 5B LD1B 2.4 99 92 81 benzoquinone 2,5-dihydroxy-3-(6-benzyloxy-1H-indol-3-yl)- 5C LD-I-214 1.7 89 97 92 [1,4]-benzoquinone 2,5-dihydroxy-3-(6-methyl-1H-indol-3-yl)-[1,4]- 5D LD10B 32.3 80 16 28 benzoquinone 2,5-dimethoxy-3-(7-fluoro-1H-indol-3-yl)-[1,4]- 5E LD-I-206 125.1 29 67 68 benzoquinone 2,5-dihydroxy-3-(7-chloro-1H-indol-3-yl)-[1,4]- 5F LD-I-207 38.0 92 23 54 benzoquinone 2,5-dihydroxy-3-(7-bromo-1H-indol-3-yl)-[1,4]- 5G LD-I-216 2.1 93 45 88 benzoquinone 2,5-dihydroxy-3-(7-methyl-1H-indol-3-yl)-[1,4]- 5H ZL-I-175 4.2 60 0 1 benzoquinone 2,5-dihydroxy-3-(7-propyl-1H-indol-3-yl)-[1,4]- 6A LD-I-215 14.1 93 23 79 benzoquinone 2,5-dihydroxy-3-(7-prenyl-1H-indol-3-yl)-[1,4]- 6B ZL-I-196 2.1 100 99 84 benzoquinone 2,5-dihydroxy-3-(7-geranyl-1H-indol-3-yl)-[1,4]- 6C LD25B 0.1 0.5 99 95 96 benzoquinone 2,5-dihydroxy-3-(7-farnecyl-1H-indol-3-yl)-[1,4]- 6D LD26B 0.1 0.5 86 89 85 benzoquinone 2,5-dihydroxy-3-(7-benzyl-1H-indol-3-yl)-[1,4]- 6E LD-I-219 1.7 98 97 98 benzoquinone 2,5-dihydroxy-3-[7-(2-methyl-benzyl)-1H-indol-3- 6F LD-I-218 0.6 1.1 90 97 94 yl]-[1,4]-benzoquinone 2,5-dihydroxy-3-(7-tert-butyl-1H-indol-3-yl)-[1,4]- 6G LD22B 2.1 96 33 37 benzoquinone 2,5-dihydroxy-3-(7-phenyl-1H-indol-3-yl)-[1,4]- 6H LD-I-143 1.4 98 92 91 benzoquinone 2,5-dihydroxy-3-(7-methoxy-1H-indol-3-yl)-[1,4]- 7A LD8B 79.5 36 15 13 benzoquinone 2,5-dihydroxy-3-(7-benzyloxy-1H-indol-3-yl)- 7B LD17B 1.6 98 97 85 [1,4]-benzoquinone 2,5-dihydroxy-3-(2,5-dimethyl-1H-indol-3-yl)- 7C ZL-I-199 16.5 61 20 25 [1,4]-benzoquinone 2,5-dihydroxy-3-(2-methyl-5-methoxy-1H-indol- 7D ZL-I-192 125.9 0 0 31 3-yl)-[1,4]-benzoquinone 2,5-dihydroxy-3-(2-methyl-5-chloro-1H-indol-3- 7E ZL-I-193 52.2 91 60 81 yl)-[1,4]-benzoquinone 2,5-dihydroxy-3-(2,6-dimethyl-1H-indol-3-yl)- 7F LD-I-209 206.7 46 16 0 [1,4]-benzoquinone 2,5-dihydroxy-3-(2,7-dimethyl-1H-indol-3-yl)- 7G LD15B 38.5 20 0 0 [1,4]-benzoquinone 2,5-dihydroxy-3-(5,6-methylenedioxy-1H-indol- 7H LD-I-125 3.7 96 88 63 3-yl)-[1,4]-benzoquinone 2,5-dihydroxy-3-(5,6-dimethoxy-1H-indol-3-yl)- 8A LD16B 116.9 39 19 20 [1,4]-benzoquinone 2,5-dihydroxy-3-(6,7-dimethyl-1H-indol-3-yl)- 8B LD-I-208 12.8 90 32 20 [1,4]-benzoquinone 2,5-dihydroxy-3-(1H-benzo[g]indol-3-yl)-[1,4]- 8C LD-I-213 2.8 98 79 75 benzoquinone 2-(2-tert-butyl-1H-indol-3-yl)-5-(7-tert-butyl-1H- 8D ZL-III-273-I 5.8 indol-3-yl)-3-chloro-6-hydroxy-[1,4]- benzoquinone DAQ-B1 8E ZL-II-241 0.9 2-(2-tert-butyl-1H-indol-3-yl)-3,6-dihydroxy-5- 8F ZL-III-268 11.2 (1H-indol-3-yl)-[1,4]-benzoquinone 2-(2-tert-butyl-1H-indol-3-yl)-3,6-dihydroxy-5-(1- 8G ZL-III-269 12.0 methyl-1H-indol-3-yl)-[1,4]-benzoquinone 2-(2-tert-butyl-1H-indol-3-yl)-3,6-dihydroxy-5-(2- 8H ZL-II-193 5.8 methyl-1H-indol-3-yl)-[1,4]-benzoquinone 2-(2-tert-butyl-1H-indol-3-yl)-3,6-dihydroxy-5-(2- 9A ZL-III-262 5.6 cyclopropyl-1H-indol-3-yl)-[1,4]-benzoquinone 2-(2-tert-butyl-1H-indol-3-yl)-3,6-dihydroxy-5-(2- 9B ZL-III-261 5.0 isopropyl-1H-indol-3-yl)-[1,4]-benzoquinone 2-(2-tert-butyl-1H-indol-3-yl)-3,6-dihydroxy-5-(2- 9C ZL-III-263 5.1 tert-butyl-1H-indol-3-yl)-[1,4]-benzoquinone 2-(2-tert-butyl-1H-indol-3-yl)-3,6-dihydroxy-5-(2- 9D ZL-III-260 6.1 phenyl-1H-indol-3-yl)-[1,4]-benzoquinone 2-(2-tert-butyl-1H-indol-3-yl)-3,6-dihydroxy-5-(4- 9E ZL-III-278 13.7 methoxy-1H-indol-3-yl)-[1,4]-benzoquinone 2-(2-tert-butyl-1H-indol-3-yl)-3,6-dihydroxy-5-(4- 9F ZL-III-277 2.3 benzyloxy-1H-indol-3-yl)-[1,4]-benzoquinone 2-(2-tert-butyl-1H-indol-3-yl)-3,6-dihydroxy-5-(5- 9G ZL-III-275 8.7 fluoro-1H-indol-3-yl)-[1,4]-benzoquinone 2-(2-tert-butyl-1H-indol-3-yl)-3,6-dihydroxy-5-(5- 9H ZL-III-270 80.3 methoxyl-1H-indol-3-yl)-[1,4]-benzoquinone 2-(2-tert-butyl-1H-indol-3-yl)-3,6-dihydroxy-5-(5- 10A ZL-III-271 0.6 benzyloxy-1H-indol-3-yl)-[1,4]-benzoquinone 2-(2-tert-butyl-1H-indol-3-yl)-3,6-dihydroxy-5-(5- 10B ZL-III-272 7.2 methyl-1H-indol-3-yl)-[1,4]-benzoquinone 2-(2-tert-butyl-1H-indol-3-yl)-3,6-dihydroxy-5-(6- 10C ZL-III-276 5.3 fluoro-1H-indol-3-yl)-[1,4]-benzoquinone 2-(2-tert-butyl-1H-indol-3-yl)-3,6-dihydroxy-5-(6- 10D ZL-III-279 5.3 methyl-1H-indol-3-yl)-[1,4]-benzoquinone 2-(2-tert-butyl-1H-indol-3-yl)-3,6-dihydroxy-5-(7- 10E ZL-III-267 4.8 methyl-1H-indol-3-yl)-[1,4]-benzoquinone 2-(2-tert-butyl-1H-indol-3-yl)-3,6-dihydroxy-5-(7- 10F ZL-III-273 0.9 tert-butyl-1H-indol-3-yl)-[1,4]-benzoquinone 2-(2-tert-butyl-1H-indol-3-yl)-3,6-dihydroxy-5- 10G ZL-III-264 14.6 (2,5-dimethyl-1H-indol-3-yl)-[1,4]-benzoquinone 2-(2-tert-butyl-1H-indol-3-yl)-3,6-dihydroxy-5-(2- 10H ZL-III-266 11.5 methyl-5-methoxy-1H-indol-3-yl)-[1,4]- benzoquinone 2-(2-tert-butyl-1H-indol-3-yl)-3,6-dihydroxy-5-(2- 11A ZL-III-265 8.4 methyl-5-chloro-1H-indol-3-yl)-[1,4]- benzoquinone 2,5-dihydroxy-3-(1-methyl-1H-indol-3-yl)-6- 11B ZL-III-185 7.9 phenyl-[1,4]-benzoquinone 2,5-dihydroxy-3,6-bis(2-methyl-1H-indol-3-yl)l- 11C ZL-III-274 15.7 [1,4]-benzoquinone 2,5-dihydoxy-3-(2-methyl-1H-indol-3-yl)- 11D ZL-III-168-II 18.0 [1,4]naphthoquinone 2,5-bis(2-tert-butyl-1H-indol-3-yl)-3-chloro-6- 11E ZL-III-263-I 22.9 hydroxy-[1,4]-benzoquinone 2-(2-tert-butyl-1H-indol-3-yl)-5-(1H-indol-3-yl)-3- 11F ZL-III-268-I 49.9 chloro-6-hydroxy-[1,4]-benzoquinone 2-(2-tert-butyl-1H-indol-3-yl)-5-(7-methyl-1H- 11G ZL-III-267-I 33.8 indol-3-yl)-3-chloro-6-hydroxy-[1,4]- benzoquinone 2-(2-tert-butyl-1H-indol-3-yl)-5-(2-phenyl-1H- 11H ZL-III-260-I 6.7 indol-3-yl)-3-chloro-6-hydroxy-[1,4]- benzoquinone

EXAMPLE 8 Inhibition of Phosphatase H1

Using procedures known to those skilled in the art, the compounds listed in Table 3 were screened against recombinant vaccinia H1 phosphatase using FMOP as a fluorogenic substrate. Compounds were initially screened at 1 micromolar concentration. Several compounds in this series completely abolish enzymatic activity at this concentration, while the majority of compounds have low enzyme inhibition activity.

The foregoing is illustrative of the present invention, and is not to be construed as limiting thereof. The invention is defined by the following claims, with equivalents of the claims to be included therein. 

1. An acid-catalyzed method of producing a compound of formula I:

wherein: R₁ and R₃ are each chlorine; R₂ is hydrogen, aryl, alkyl, alkoxy, phenoxy, anilino, amino, halo, acyloxy, or (acyloxy)alkyl; R₅ is hydrogen, C₁–C₇ alkyl, C₂–C₇ alkenyl, C₂–C₇ alkynyl, arylalkyl, or aryl; R₄ is, alkylcarboxy, C₂–C_(m) alkenyl, alkynyl, alkenylcarboxy, hydroxy, hydroxyalkyl, C₁–C_(n) alkoxy, nitro, halo, trihalomethyl, amido, carboxamido, carboxy, sulfonyl, sulfonamido, amino, mercapto, or 2-methylbut-2-en-4-yl, wherein n is an integer from 2–12, and m is an integer from 3–12; and R₆ and R₇ form part of an aromatic ring wherein said aromatic ring may be substituted; which method comprises: reacting a substituted or unsubstituted 2,5-dichloro-1,4-benzoquinone compound of formula II:

wherein R₁, R₂ and R₃ are as defined above; with at least one pyrrole of the formula III:

wherein R₄–R₇ are as defined above; in a polar organic solvent and in the presence of an acid to produce a first intermediate; and then reacting the first intermediate with an oxidization agent to produce said compound of formula I.
 2. The method of claim 1, wherein n is 2–7 and m is 3–7.
 3. The method of claim 1, wherein the organic solvent is an aprotic solvent selected from the group consisting of tetrahydrofuran (THF), acetonitrile, and mixtures thereof.
 4. The method of claim 1, wherein the acid is HCl.
 5. The method of claim 1, wherein the acid is H₂SO₄.
 6. The method of claim 1, wherein the acid is AcOH.
 7. The method of claim 1, wherein the oxidization agent is dichlorodicyanobenzoquinone.
 8. The method of claim 1, wherein the oxidization agent is Ag₂CO₃.
 9. The method of claim 1, wherein the reaction is conducted at a temperature from about −10° C. to about 100° C. 